Abstract
Small lymphocytes represent a mixed population with varying life spans (Ottesen, 1964; Everett et al., 1964; Robinson et al., 1965). In the present report evidence will be presented indicating that at least two populations of lymphoid cells with different tinctorial properties and unequal DNA-synthesis and generation times exist in the thoracic duct of calves. The data are based on a combined analysis of the mitotic labeling index (MLI) and the mean grain count per labeled mitotic figure (MGC/LMF), both as a function of time after a single intravenous injection of Thymidine-3H. This method was previously found to necessitate the least number of assumptions in evaluating proliferative patterns of cell lines without detectable cytoplasmic differentiation (Janett et al., 1966).
Supported by the U.S Atomic Energy Commission and the Swiss National Foundation for Scientific Research.
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Safier, S., Wagner, H.P., Cottier, H., Rai, K., Jansen, C.R., Cronkite, E.P. (1967). Lymphoid Cell Lines in the Thoracic Duct of the Calf with Different Generation Times. In: Cottier, H., Odartchenko, N., Schindler, R., Congdon, C.C. (eds) Germinal Centers in Immune Responses. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-86837-5_20
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DOI: https://doi.org/10.1007/978-3-642-86837-5_20
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