Abstract
Small lymphocytes represent a mixed population with varying life spans (Ottesen, 1964; Everett et al., 1964; Robinson et al., 1965). In the present report evidence will be presented indicating that at least two populations of lymphoid cells with different tinctorial properties and unequal DNA-synthesis and generation times exist in the thoracic duct of calves. The data are based on a combined analysis of the mitotic labeling index (MLI) and the mean grain count per labeled mitotic figure (MGC/LMF), both as a function of time after a single intravenous injection of Thymidine-3H. This method was previously found to necessitate the least number of assumptions in evaluating proliferative patterns of cell lines without detectable cytoplasmic differentiation (Janett et al., 1966).
Supported by the U.S Atomic Energy Commission and the Swiss National Foundation for Scientific Research.
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Cottier, H., N. Odartchenko, G. Reiser, M. Hess, and R. D. Stoner: Incorporation of tritiated nucleosides and amino acids into lymphoid and plasmacytoid cells during secondary response to tetanus toxoid in mice. Ann. N.Y. Acad. Sci. 113, 612–626 (1964).
Cronkite, E. p., C. R. Jansen, G. Mather, N. Nielsen, E. A. Usenik, E. R. Adamik, and C. R. Sipe: Studies on lymphocytes. I. Lymphopenia produced by prolonged extracorporeal irradiation of circulating blood. Blood 20, 203–213 (1962).
Everett, N. B., R. W. Caffrey, and W. O. Rieke: Recirculation of lymphocytes. Ann. N.Y. Acad. Sci. 113, 887–897 (1964).
Fliedner, T. M., M. Kesse, E. P. Cronkite, and J. S. Robertson: Cell proliferation in germinal centers of the rat spleen. Ann. N.Y. Acad. Sci. 113, 578–594 (1964).
Hanna, M. G. Jr.: An autoradiographic study of the germinal center in spleen white pulp during early intervals of the immune response. Lab. Invest. 13, 95–104 (1964).
Janett, a., H. p. Wagner, C. R. Jansen, H. Cottier, and E. P. Cronkite: Studies on lymphopoiesis. IV. a comparison of two approaches for the determination of the generation time in thoracic duct cells without detectable cytoplasmic differentiation. Europ. J. Cancer 2, 231–236 (1966).
Michalke, M.: Personal communication.
Ottesen, J.: On the age of human white cells in peripheral blood. Acta Physiol. Scand. 32, 75–93 (1954).
Robinson, S. H., G. Brecher, I. S. Lourie, and J. E. Haley: Leukocyte labeling in rats during and after continuous infusion of tritiated thymidine: Implications of lymphocyte longevity and DNA reutilization. Blood 26, 281–295 (1965).
Slatkin, D. N., C. R. Jansen, E. P. Cronkite, and J. S. Robertson: Extracorporeal irradiation of blood: Calculations of the radiation dose. Radiation Res. 19, 409–418 (1963).
Wagner, H. P., H. Cottier, E. P. Cronkite, L. Cunningham, C. R. Jansen, and K. Rai: Studies on lymphocytes. V. Short in-vivo DNA-synthesis and generation time of lymphoid cells in the calf thoracic duct after simulated or effective extra-corporeal irradiation of circulating blood (in preparation).
References
Astaldi, G., M. Gociu, and R. Airo: Unpublished data.
Braunsteiner, H., R. Hofer, and S. Sailer: The lymphocyte. Germ. med. Mth. 6, 273–276 (1961).
Davies, A. J. S., E. Leuchars, V. Wallis, R. Marchant, and E. V. Elliott: The failure of thymus-derived cells to produce antibody. Transplantation. (In press.)
Sainte-Marie, G., and C. P. Leblond: Elaboration of a model for the formation of lymphocytes in the thymic cortex. Blood 26, 765–783 (1965).
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Safier, S., Wagner, H.P., Cottier, H., Rai, K., Jansen, C.R., Cronkite, E.P. (1967). Lymphoid Cell Lines in the Thoracic Duct of the Calf with Different Generation Times. In: Cottier, H., Odartchenko, N., Schindler, R., Congdon, C.C. (eds) Germinal Centers in Immune Responses. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-86837-5_20
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DOI: https://doi.org/10.1007/978-3-642-86837-5_20
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