Skip to main content

Cryopreservation of Germplasm of Medicinal and Aromatic Plants

  • Chapter
Cryopreservation of Plant Germplasm I

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 32))

Abstract

Plants are the most important source of medicines (Balandrin and Klocke 1988); however, due to pressures of human population and economic development, the natural ecosystem is continually disturbed throughout the world. This has resulted in the depletion of naturally occurring genetic resources and germplasm pools from which future pharmaceutical and other useful products might be developed. This has caused international concern for the conservation of germ-plasm. There is an urgent need to conserve the germplasm of thousands of rare, elite, and endangered species of medicinal plants, especially those of recalcitrant types in which seed cannot be preserved. In this regard in vitro culture has played a significant role, and now there are numerous species of medicinal and aromatic plants belonging to diverse families on which micropropagation studies have been conducted. Bajaj et al. (1988) reviewed the subject that deals with 148 such species belonging to 108 genera.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bajaj YPS (1976a) Regeneration of plants from cell suspensions frozen at — 20, — 70, and —196° C. Physiol Plant 37: 263–268

    Article  Google Scholar 

  • Bajaj YPS (1976b) Gene preservation through freeze storage of cell, tissue and organ cultures.Acta Hortic 63: 75–84

    Google Scholar 

  • Bajaj YPS (1977) Survival of Atropa and Nicotiana pollen embryos frozen at —196° C. Curr Sci 46: 305–307

    Google Scholar 

  • Bajaj YPS (1978) Effect of superlow temperature on excised anthers and pollen embryos of Atropa, Nicotiana and Petunia. Phytomorphology 28: 171–176

    Google Scholar 

  • Bajaj YPS (1981) Regeneration of plants from ultra-low frozen anthers of Primula obconica. Sci Hortic 14: 93–95

    Article  Google Scholar 

  • Bajaj YPS (1984) Induction of growth in frozen embryos of coconut and ovules of citrus. Curr Sci 53: 1215–1216

    Google Scholar 

  • Bajaj YPS (1985a) Cryopreservation of germplasm of potato (Solanum tuberosum L.) and cassava (Manihot esculenta Crantz): viability of excised meristems cryopreserved up to four years. Indian J Exp Biol 23: 285–287

    Google Scholar 

  • Bajaj YPS (1985b) Cryopreservation of embryos. In: Kartha KK (ed) Cryopreservation of plant cells and organs. CRC, Boca Raton, pp 227–242

    Google Scholar 

  • Bajaj YPS (1986) In vitro preservation of genetic resources—techniques and problems. Int Symp Nuclear techniques and in vitro culture for plant improvement. IAEA/FAO, Vienna, 1985, pp 43–57

    Google Scholar 

  • Bajaj YPS (1987) Cryopreservation of pollen and pollen embryos, and the establishment of pollen banks. In: Giles KL, Prakash J (eds) Pollen development and cytology. Academic Press, London, pp 397–420

    Google Scholar 

  • Bajaj YPS (1988a) Cryopreservation and the retention of biosynthetic potential in cell cultures of medicinal and alkaloid-producing plants. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4. Medicinal and aromatic plants I. Springer, Berlin Heidelberg New York, pp 169–197

    Google Scholar 

  • Bajaj YPS (1988b) Regeneration of plants from frozen (— 196 °C) protoplasts of Atropa belladonna L., Datura innoxia Mill. and Nicotiana tabacum L. Indian J Exp Biol 26: 289–292

    Google Scholar 

  • Bajaj YPS (1989) Cryopreservation of plant protoplasts. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 8. Plant protoplasts and genetic engineering I. Springer, Berlin Heidelberg New York, pp 97–106

    Google Scholar 

  • Bajaj YPS (ed) (1990) Biotechnology in agriculture and forestry, vol 11. Somaclonal variation in crop improvement I. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Bajaj YPS (1991) Storage and cryopreservation of in vitro cultures. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 17. High-tech and micropropagation I. Springer, Berlin Heidelberg New York, pp 361–381

    Google Scholar 

  • Bajaj YPS (1993) A suggested method for in vitro long-term storage at 4 °C of Chrysanthemum and Petunia germplasm. Plant Tissue Cult 3 (1): 57–58

    Google Scholar 

  • Bajaj YPS (1995) Cryopreservation of somatic embryos. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 30. Somatic embryogenesis and synthetic seed I. Springer, Berlin Heidelberg New York, pp 221–229

    Google Scholar 

  • Bajaj YPS, Gosch G, Ottma M, Weber A, Gröbler A (1978) Production of polyploid and aneuploid plants from anthers and mesophyll protoplasts of Atropa belladonna and Nicotiana tabacum. Indian J Exp Biol 16: 947–953

    Google Scholar 

  • Bajaj YPS, Furmanowa M, Olszowska 0 (1988) Biotechnology of the micropropagation of medicinal and aromatic plants. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4. Medicinal and aromatic plants I. Springer, Berlin Heidelberg New York, pp 60–103

    Google Scholar 

  • Balandrin MF, Klocke JA (1988) Medicinal, aromatic, and industrial materials from plants. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4. Medicinal and aromatic plants I. Springer, Berlin Heidelberg New York, pp 3–36

    Google Scholar 

  • Benson EE, Hamill JD (1991) Cryopreservation and post freeze molecular and biosynthetic stability in transformed roots of Beta vulgaris and Nicotiana rustica. Plant Cell Tissue Organ Cult 24: 163–172

    Article  CAS  Google Scholar 

  • Bertrand-Desbrunais AJ, Fabre J, Engelmann F, Dereuddre J, Charrier A (1989) Reprise de l’embryogenèse adventive d’ embryons somatiques de caféier (Coffea arabica) après congélation dans l’azote liquide. CR Acad Sci Paris 307, Ser III: 795–801

    Google Scholar 

  • Braun A (1988) Cryopreservation of sugarbeet germplasm. Plant Cell Tissue Organ Cult 14: 161–168

    Article  Google Scholar 

  • Butenko RG, Popov AS, Volkova LA, Chernyak ND, Nosov AM (1984) Recovery of cell cultures and their biosynthetic capacity after storage of Dioscorea deltoidea and Panax ginseng cells in liquid nitrogen. Plant Sci Lett 33: 285–292

    Article  CAS  Google Scholar 

  • Chaudhury R, Radhamani J, Chandel KPS (1991) Preliminary observations on the cryopreservation of desiccated embryonic axes of tea [Camellia sinensis (L.) O. Kuntzel seeds for genetic conservation. Cryo-Lett 12: 31–36

    Google Scholar 

  • Cellàrovà E, Cernicka T, Vranova E, Brutovska R, Lapar M (1992) Viability of Chamomilla recutita L. Ruaschert cells after cryopreservation. Cryo-Lett 13: 37–42

    Google Scholar 

  • Chen THH, Kartha KK, Leung NL, Kurz WGW, Chatson KB, Constabel F (1984) Cryopreservation of alkaloid-producing cell cultures of periwinkle (Catharanthus roseus). Plant Physiol 75: 726–731

    Article  PubMed  CAS  Google Scholar 

  • De-Eknamkul W, Ellis BE (1988) Rosmarinic acid: production in plant cell cultures. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4. Medicinal and aromatic plants I. Springer, Berlin Heidelberg New York, pp 310–329

    Google Scholar 

  • Demeulemeester MAC, Vandenbussche B, de Profit MP (1993) Regeneration of chicory plants from cryopreserved in vitro shoot tips. Cryo-Lett 14 (1): 57–64

    Google Scholar 

  • Denchev PD, Kuklin AI, Scragg AH (1992) Somatic embryo production in bioreactors. J Biotech 26: 99–109

    Article  CAS  Google Scholar 

  • Dereuddre J, Galerne M, Gazeau C (1987) Effets du saccharose sur la résistance à la congélation dans l’azote liquide (-196 °C) des méristèmes d’Oeillet (Dianthus caryophyllus L.) cultivés in vitro. CR Acad Sci Paris 304: 485–488

    CAS  Google Scholar 

  • Dereuddre J, Fabre J, Bassaglia C (1988) Resistance to freezing in liquid nitrogen of carnation (Dianthus caryophyllus L. var. Eolo) axillary shoot-tips excised from different aged in vitro plantlets. Plant Cell Rep 7: 170–173

    Google Scholar 

  • Diettrich B, Popov AS, Pfeiffer B, Neumann D, Butenko R, Luckner M (1982) Cryopreservation of Digitalis lanata cell cultures. Planta Med 46: 82–87

    Article  PubMed  CAS  Google Scholar 

  • Diettrich B, Haack U, Luckner M (1986) Cryopreservation of Digitalis lanata cells grown in vitro. Precultivation and recultivation. J Plant Physiol 126: 63–73

    Google Scholar 

  • Ellis D (1993) Transformation in spruce (Picea species). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 23. Plant protoplasts and genetic engineering IV. Springer, Berlin Heidelberg New York, pp 315–330

    Google Scholar 

  • Endo T, Hamaguchi N, Eriksson T, Yamada Y (1991) Alkaloid biosynthesis in somatic hybrids of Duboisia leichhardtii F. Muell. and Nicotiana tabacum L. Planta 183: 505–510

    Article  CAS  Google Scholar 

  • Fabre J, Dereuddre J (1987) Effects of different sugars (sucrose, glucose, sorbitol and mannitol) on the resistance to deep freezing in liquid nitrogen of meristems from in vitro cultured carnations (Dianthus caryophyllus L. var Eolo). CR Acad Sci Paris 304: 507–510

    CAS  Google Scholar 

  • Fett-Neto A, DiCosmo F, Reynolds WF, Sakata K (1992) Cell culture of Taxus as a source of the antineoplastic drug taxol and related taxanes. Biotechnology 10: 1572–1575

    Article  PubMed  CAS  Google Scholar 

  • Fujita Y, Takahashi S, Yamada Y (1985) Selection of cell lines with high productivity of shikonin derivatives by protoplast culture of Lithospermum erythrorhizon cells. Agric Biol Chem 49: 1755–1759

    Article  CAS  Google Scholar 

  • Fukai S (1989) Plant regeneration from shoot tips of Dianthus hybrida cryopreserved in liquid nitrogen up to 2 years. Plant Tissue Cult Lett 6: 177–178

    Article  Google Scholar 

  • Fukai S (1990) Cryopreservation of chrysanthemum shoot tips. Sci Hortic 45: 167–174

    Article  Google Scholar 

  • Fukai S, Goi M, Tanaka M (1991) Cryopreservation of shoot tips of Chrysanthemum morifolium and related species native to Japan. Euphytica 54: 201–204

    Google Scholar 

  • Gleba YY, Hinnisdaels S, Sidorov VA, Kaleda VA, Parakonny AS, Boryshuk NV, Cherep NN, Negrutiu I, Jacobs M (1988) Intergeneric asymmetric hybrids between Nicotiana plumbaginifolia and Atropa belladonna obtained by gamma-fusion. Theor Appl Genet 76: 760–766

    Article  Google Scholar 

  • Göldner EM, Seitz U, Reinhard E (1991) Cryopreservation of Digitalis lanata Ehrh. cell cultures: preculture and freeze tolerance. Plant Cell Tissue Organ Cult 24: 19–24

    Article  Google Scholar 

  • Gosch G, Bajaj YPS, Reinert J (1975) Isolation, culture and induction of embryogenesis in protoplasts from cell-suspensions of Atropa belladonna. Protoplasma 86: 405–410

    Article  CAS  Google Scholar 

  • Hashimoto T, Azechi S (1988) Bioreactors for the large-scale culture of plant cells. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4. Medicinal and aromatic plants I. Springer, Berlin Heidelberg New York, pp 104–122

    Google Scholar 

  • Hauptmann RM, Widholm JM (1982) Cryostorage of cloned amino acid analog resistant carrot and tobacco suspension culture. Plant Physiol 70: 30–37

    Article  PubMed  CAS  Google Scholar 

  • Hiraoka N (1988) Nonfrozen storage of plant cell cultures and its effect on metabolites. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4. Medicinal and aromatic plants I. Springer, Berlin Heidelberg New York, pp 157–168

    Google Scholar 

  • Hunter CS (1986) In vitro propagation and germplasm storage of Cinchona. In: Withers L, Alderson P, (eds) Plant tissue culture and its agricultural applications. Butterworth, London, pp 291–301

    Google Scholar 

  • Ikuta A, Itokawa H (1988) Berberine: production through plant (Thalictrum spp.) cell cultures. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4. Medicinal and aromatic plants I. Springer, Berlin Heidelberg New York, pp 282–293

    Google Scholar 

  • Inomata S, Yokoyama M, Gozu Y, Shimizu T, Yanagi M (1993) Growth pattern and ginsenoside production of Agrobacterium-transformed Panax ginseng roots. Plant Cell Rep 12: 681–686

    Article  CAS  Google Scholar 

  • Ishimaru K, Sudo H, Satke M, Shimomura K (1990) Phenyl glucosides from a hairy root culture of Swertia japonica. Phytochemistry 29: 3823–3825

    Article  CAS  Google Scholar 

  • Jekkel ZS, Heszky LE, Ali AH (1989) Effect of different cryoprotectants and transfer temperatures on the survival rate of hemp (Cannabis sativa L.) cell suspension in deep freezing. Acta Biol Hung 40: 127–136

    PubMed  CAS  Google Scholar 

  • Kobayashi S, Sakai A, Oiyama I (1990) Cryopreservation in liquid nitrogen of cultured navel orange (Citrus sinensis Osb.) nucellar cells and subsequent plant regeneration. Plant Cell Tissue Organ Cult 23: 15–20

    Article  Google Scholar 

  • Kostenyuk IA, Lubaretz O, Borisyuk N, Voronin V, Cherep N, Stockigt J, Gleba Y (1991) Isolation and characterization of intergeneric somatic hybrids in Apocynaceae family. Theor Appl Genet 82: 713–716

    Article  Google Scholar 

  • Krumbiegel G, Schieder 0 (1979) Selection of somatic hybrids after fusion of protoplasts from Datura innoxia Mill. and Atropa belladonna L. Planta 145: 371–376

    Google Scholar 

  • Kuriyama A, Watanabe K, Ueno S, Mitsuda H (1990) Effect of post-thaw treatment on the viability of cryopreserved Lavandula vera cells. Cryo-Lett 11: 171–178

    Google Scholar 

  • Kushnir S, Babiychuk E, Bannikova M, Momot V, Komarnitsky I, Cherep N, Gleba Y (1991) Nucleo-cytoplasmic incompatibility in cybrid plants possessing an Atropa genome and a Nicotiana plastome. Mol Gen Genet 225: 225–230

    Article  PubMed  CAS  Google Scholar 

  • Lazar (1989) Somatic hybridization in Hyoscyamus x Nicotiana. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 8. Plant protoplasts and genetic engineering I. Springer, Berlin Heidelberg New York, pp 356–369

    Google Scholar 

  • Maddox AD, Gonsalves F, Shields R (1983) Successful preservation of suspension cultures of three Nicotiana species at the temperature of liquid nitrogen. Plant Sci Lett 28: 157–162

    Google Scholar 

  • Marin ML, Duran-Vila N (1988) Survival of somatic embryos and recovery of plants of sweet orange [Citrus sinensis ( L.) Osb.] after immersion in liquid nitrogen. Plant Cell Tissue Organ Cult 14: 51–57

    Google Scholar 

  • Merkle SA, Schlarbaum SE, Cox RA, Schwarz OJ (1991) Mass propagation of somatic embryo-derived plantlets of yellow-poplar for field testing. In: Proc 21st Conf Southern Forest tree improvement Knoxville, TN, pp 56–68

    Google Scholar 

  • Morris P, Robbins MP (1992) Condensed tannin formation by Agrobacterium rhizogenes transformed root and shoot organ cultures of Lotus corniculatus. J Exp Bot 43: 221–231

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Nag KK, Street HE (1975) Freeze-preservation of cultured plant cells. II. The freezing and thawing phases. Physiol Plant 34: 261–265

    Google Scholar 

  • Niwata E (1992) Cryopreservation of shoot tips of garlic by vitrification. Jpn J Breed (Suppl 1 ): 326

    Google Scholar 

  • Ogino T, Hiraoka N, Tabata M (1978) Selection of high nicotine producing cell lines of tobacco callus by single cell cloning. Phytochemistry 17: 1907–1910

    Article  CAS  Google Scholar 

  • Pence VC (1991) Cryopreservation of immature embryos of Theobroma cacao. Plant Cell Rep 10: 144–147

    Article  Google Scholar 

  • Radhamani J, Chandel KPS (1992) Cryopreservation of embryonic axes of trifoliate orange (Poncirus trifoliata L. RAF.). Plant Cell Rep 11: 372–374

    Google Scholar 

  • Redenbaugh K, Fujii J, Slade D, Viss P, Kossler M (1991) Artificial seeds — encapsulated somatic embryos. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 17. High-tech and micropropagation I. Springer, Berlin Heidelberg New York, pp 395–416

    Google Scholar 

  • Reichling J, Thron U (1990) Accumulation of rare phenylpropanoids in Agrobacterium rhizogenes transformed root cultures of Coreopsis tinctoria. Planta Med 56: 488–490

    Article  PubMed  CAS  Google Scholar 

  • Reuff I, Seitz U, Ulrich B, Reinhard E (1988) Cryopreservation of Coleus blumei suspension and callus cultures. J Plant Physiol 133: 414–418

    Article  Google Scholar 

  • Sakai A, Kobayashi S, Oyiama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var, brasiliensis Tanaka) by vitrification. Plant Cell Rep 9: 30–33

    Google Scholar 

  • Sato F, Yamada Y (1984) High berberine-producing cultures of Coptis japonica cells. Phytochemistry 23: 281–285

    Article  CAS  Google Scholar 

  • Seibert M (1976) Shoot initiation from carnation shoot apices frozen to —196 °C. Science 191: 1178–1179

    Article  PubMed  CAS  Google Scholar 

  • Seitz U, Reinhard E (1987) Growth and ginsenoside pattern of cryopreserved Panax ginseng cell cultures. J Plant Physiol 131: 215–223

    Article  CAS  Google Scholar 

  • Seitz U, Alfermann AW, Reinhard E (1983) Stability of biotransformation capacity in Digitalis lanata cell cultures after cryogenic storage. Plant Cell Rep 2: 273–276

    Article  CAS  Google Scholar 

  • Sim SJ, Chang HN (1993) Increased shikonin production by hairy roots of Lithospermum erythrorhizon in two phase bubble column reactor. Biotechnol Lett 15 (2): 145–150

    Article  CAS  Google Scholar 

  • Sugawara Y, Sakai A (1974) Survival of suspension-cultured sycamore cells cooled to the temperature of liquid nitrogen. Plant Physiol 54: 722–724

    Article  PubMed  CAS  Google Scholar 

  • Taniguchi K, Tanaka R, Ashitani N, Miyagawa H (1988) Freeze preservation of tissue-cultured shoot primordia of the annual Haplopappus gracilis (2n = 4). Jpn J Genet 63: 267–272

    Article  Google Scholar 

  • Tannoury M, Ralambosoa J, Kaminski M, Dereuddre J (1991) Cryoconservation par vitrification d’apex enrobés d’oeillet (Dianthus caryophyllus L.) cultivé in vitro. CR Acad Sci, Paris Sér III, 313: 633–638

    Google Scholar 

  • Tessereau H, Lecouteux C, Florin B, Schlienger C, Petiard V (1991) Use of a simplified freezing process and dehydration for the storage of embryogenic cell lines and somatic embryos. Rev Cytol Biol Veg Bot 14: 297–310

    Google Scholar 

  • Towill LE (1990) Cryopreservation of isolated mint shoot tips by vitrification. Plant Cell Rep 9: 178–180

    Article  Google Scholar 

  • Uemura M, Sakai A (1980) Survival of carnation (Dianthus caryophyllus L.) shoot apices frozen to the temperature of liquid nitrogen. Plant Cell Physiol 21: 85–94

    CAS  Google Scholar 

  • Watanabe K, Mitsuda H, Yamada Y (1983) Retention of metabolic and differentiation potential of green Lavandula vera callus after freeze-preservation. Plant Cell Physiol 24: 119–122

    CAS  Google Scholar 

  • Weber G, Roth EJ, Schweiger HG (1983) Storage of cell suspensions and protoplasts of Glycine max (L.) Merr, Brassica napus (L.), Datura innoxia (Mill.), and Daucus carota ( L.) by freezing. Z Pflanzenphysiol 109: 29–39

    Google Scholar 

  • Wesley-Smith J, Vertucci CW, Berjak P, Pammenter NW, Crane J (1992) Cryopreservation of desiccation-sensitive axes of Camellia sinensis in relation to dehydration, freezing rate and the thermal properties of tissue water. J Plant Physiol 140: 596–604

    Article  Google Scholar 

  • Yamamoto Y, Mizuguchi R, Yamada Y (1982) Selection of a high stable pigment-producing strain in cultured Euphorbia millii cells. Theor Appl Genet 61: 113–116

    Article  CAS  Google Scholar 

  • Yamamoto H, Ieda K, Tsuchiya SI, Yan K, Tanaka T, linuma M, Mizuno M (1992) Flavonol

    Google Scholar 

  • glycoside production in callus cultures of Epimedium diphyllum. Phytochemistry 31: 837–840 Yoshimatsu K, Yamaguchi H, Shimomura K (1995) Cold storage and cryopreservation of hairy roots of Panax ginseng. Plant Cell Rep (in press)

    Google Scholar 

  • Zhang LX, Chang WC, Wei YJ, Liu L, Wang YP (1993) Cryopreservation of ginseng pollen. HortScience 28 (7): 742–743

    Google Scholar 

  • Zheng Guang-zhi, He Jing-bo, Wang Shi-ling (1983) Cryopreservation of calli and their suspension culture cells of Anisodus acutangulus. Acta Bot Sin 25 (6): 512–517 (in Chinese)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Bajaj, Y.P.S. (1995). Cryopreservation of Germplasm of Medicinal and Aromatic Plants. In: Bajaj, Y.P.S. (eds) Cryopreservation of Plant Germplasm I. Biotechnology in Agriculture and Forestry, vol 32. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03096-7_29

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-03096-7_29

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08184-2

  • Online ISBN: 978-3-662-03096-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics