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Regeneration of Plants from Protoplasts of Poa pratensis L. (Kentucky Blue Grass)

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Plant Protoplasts and Genetic Engineering VII

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 38))

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Abstract

Poa pratensis (Kentucky blue grass) is a cool-season grass crop, important both for turf and fodder purposes. Since it tolerates foot traffic and provides a tight, firm surface, Poa pratensis is widely used for the maintenance of athletic fields and golf courses in Canada, the USA, and Europe (Beard 1973). The fodder cultivars are characterized by their high dry matter production. In nature, wild-type Kentucky blue grasses were originally found in Europe and Asia. It is nowadays a common species throughout the cool-season areas in both the North and South hemispheres. The grass is present at both high and low latitudes, and grows on both moist and dry soils. The accumulation of dry matter, and tillering and height growth of various ecotypes have been shown to be strongly adapted to their place of origin (Aamlid 1992). An improved relative growth rate of, e.g., high-latitude ecotypes, has been accomplished by the application of longer photoperiods (Heide et al. 1985).

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References

  • Aamlid TS (1992) Effects of temperature and photoperiod on growth and development of tillers and rhizomes in Poa pratensis L. ecotypes. Ann Bot 69: 289–292

    Google Scholar 

  • Akerberg E (1939) Apomictic and sexual seed formation in Poa pratensis. Hereditas 25: 359–370 Beard JB (1973) Turfgrass: science and culture. Prentice Hall, Englewood Cliffs

    Google Scholar 

  • Boyd LA, Dale PJ (1986) Callus production and plant regeneration from mature embroys of Poa pratensis L. Plant Breed 97: 246–254

    Article  CAS  Google Scholar 

  • Frearson EM, Power JB, Cocking EC (1973) The isolation, culture and regeneration of Petunia leaf protoplasts. Dev Biol 33: 130–137

    Article  PubMed  CAS  Google Scholar 

  • Funatsuki H, Lörz H, Lazzeri PA (1992) Use of feeder cells to improve barley protoplast culture and regeneration. Plant Sci 85: 179–187

    Article  Google Scholar 

  • Grazi F, Umaerus M, Akerberg E (1961) Observation on the mode of reproduction and the embryology of Poa pratensis. Hereditas 47: 489–541

    Article  Google Scholar 

  • Heide OM, Bush MG, Evans LT (1985) Interaction of photoperiod and gibberellin on growth and photosynthesis of high-latitude Poa pratensis. Physiol Plant 65: 135–145

    Article  CAS  Google Scholar 

  • Jähne A, Lazzeri PA, Jäger-Gussen M, Lrz H (1991) Plant regeneration from embryogenic cell suspensions derived from anther cultures of barley (Hordeum vulgare L.). Theor Appl Genet 82: 74–80

    Article  Google Scholar 

  • Kao KN, Michyluk MC (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126: 105–110

    Article  CAS  Google Scholar 

  • Kyozuka J, Hayashi Y, Shimamoto K (1987) High frequency plant regeneration from rice protoplasts by novel culture methods. Mol Gen Genet 206: 408–413

    Article  CAS  Google Scholar 

  • Lührs R, Lrz H (1988) Initiation of morphogenic cell suspensions and protoplast cultures of barley (Hordeum vulgare L.). Planta 175: 71–81

    Article  Google Scholar 

  • Lührs R, Nielsen KA (1992) Microspore cultures as donor tissue for the initiation of embryogenic cell suspensions of barley (Hordeum vulgare L.). Plant Cell Tissue Organ Cult 31: 169–178

    Article  Google Scholar 

  • McDonnell RE, Conger BV (1984) Callus induction and plantlet formation from mature embryo explants of Kentucky bluegrass. Crop Sci 24: 573–578

    Article  Google Scholar 

  • Nielsen KA, Knudsen E (1993) Regeneration of green plants from embryogenic suspension cultures of Kentucky blue grass (Poa pratensis L.). J Plant Physiol 141: 589–595

    Article  Google Scholar 

  • Nielsen KA, Larsen E, Knudsen E (1993) Regeneration of protoplast-derived green plants of Kentucky blue grass (Poa pratensis L.). Plant Cell Rep 12: 537–540

    Article  CAS  Google Scholar 

  • Potrykus I, Harms CT, Lörz H (1979) Callus formation from cell culture protoplasts of corn (Zea mays L.). Theor Appl Genet 54: 209–214

    Article  Google Scholar 

  • Van Ark HF, Zaal MACM, Creemers-Molenaar J, van der Valk P (1991) Improvement of the tissue culture response of seed-derived callus cultures of Poa pratensis L.: effect of gelling agent and abscisic acid. Plant Cell Tissue Organ Cult 7: 275–280

    Article  Google Scholar 

  • Van der Valk P, Zall MACM, Creemers-Molenaar J (1988) Regeneration of albino plantlets from suspension culture drived protoplasts of Poa pratensis L. ( Kentucky blue grass ). Euphytica: 169–176

    Google Scholar 

  • Van der Valk P, Zaal MACM, Creemers-Molenaar J (1989) Somatic embryogenesis and plant regeneration of inflorescence and seed derived callus cultures of Poa pratensis L. ( Kentucky bluegrass ). Plant Cell Rep 7: 644–647

    Google Scholar 

  • Van Dreven F, Esselink G, Houwing A (1990) Esterase isozyme differences between cultivars of Kentucky bluegrass (Poa pratensis L.) from seed extracts using isoelectric focusing. Plant varieties and Seeds 3: 89–97

    Google Scholar 

  • Vasil V, Vasil IK (1986) Plant regeneration from friable embryogenic callus and cell suspension cultures of Zea mays L. J Plant Physiol 124: 399–408

    Article  CAS  Google Scholar 

  • Vasil V, Redway F, Vasil IK (1990) Regeneration of plants from embryogenic suspension culture protoplasts of wheat (Triticum aestivum L.). Bio Technology 8: 429–434

    Article  Google Scholar 

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© 1996 Springer-Verlag Berlin Heidelberg

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Nielsen, K.A. (1996). Regeneration of Plants from Protoplasts of Poa pratensis L. (Kentucky Blue Grass). In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering VII. Biotechnology in Agriculture and Forestry, vol 38. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-09368-9_11

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  • DOI: https://doi.org/10.1007/978-3-662-09368-9_11

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08240-5

  • Online ISBN: 978-3-662-09368-9

  • eBook Packages: Springer Book Archive

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