Skip to main content

Regeneration of Plants from Protoplasts of Saintpaulia ionantha H. Wendl. (African Violet)

  • Chapter
Plant Protoplasts and Genetic Engineering VII

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 38))

  • 236 Accesses

Abstract

The African violet was described scientifically for the first time in 1893, and named Saintpaulia ionantha by Wendland (1893). The genus belongs to the family Gesneriaceae, and the 20 species originate from South-East Africa. Breeding was done mainly with Saintpaulia ionantha and S. confusa, and as a result the cultivars existing today are regarded as S. ionantha hybrids (Moore 1957). Intensive breeding efforts led to a wide spectrum of cultivars in different flower colors and shapes. The economic importance of S. ionantha as an ornamental warmhouse plant increased, so now it is one of the most-sold plants in Europe and the USA. However, important characters have to be improved, like plant quality with regard to transport ability, cold tolerance, disease and pest resistances, especially against the Californian flower thrips (Frankliniella occidentalis),and yellow, orange, or true red flower colors.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bilkey PC (1978) The microculture of two members of the Gesneriaceae, Saintpaulia and Episcia, and the prospects for asexual hybridisation. Thesis, Univ Wisconsin-Madison

    Google Scholar 

  • Bilkey PC, Cocking EC (1982) A non-enzymatic method for the isolation of protoplasts from callus of Saintpaulia ionantha ( African Violet ). Z Pflanzen Physiol 105: 285–288

    Google Scholar 

  • Bilkey PC, McCown BH (1978) Towards true red, orange and yellow-flowering African violets — asexual hybridisation of Saintpaulia and Episcia. Afr Viol Mag 31: 64–66

    Google Scholar 

  • Damm B, Willmitzer L (1988) Regeneration of fertile plants from protoplasts of different Arabidopsis thaliana genotypes. Mol Gen Genet 213: 15–20

    Article  Google Scholar 

  • Draget KJ, Myhre S, Skjâk-Bræk, Ostgaard K (1988) Regeneration, cultivation and differentiation of plant protoplasts immobilized in Ca-alginate beads. J Plant Physiol 321: 552–556

    Article  Google Scholar 

  • Evans PK, Cocking EC (1975) The technique of plant cell culture and somatic cell hybridization. In: Pain RH, Smith BJ (eds) New techniques in biophysics and cell biology, vol 2. London, pp 127–158

    Google Scholar 

  • Fernandez M, Torres KC (1984) Isolation of protoplasts from leaf mesophyll in African violet. HortScience 19 (3 Sect 2): 555

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158

    Article  PubMed  CAS  Google Scholar 

  • Grunewaldt J (1977) Adventivknospenbildung und Pflanzenregeneration bei Gesneriaceae in vitro. Gartenbauwissenchaft 42 (4): 171–175

    Google Scholar 

  • Hoshino Y, Nakano M, Mii M (1995) Plant regeneration from cell suspension derived protoplasts of Saintpaulia ionantha Wendl. Plant Cell Rep 14: 341–344

    Article  CAS  Google Scholar 

  • Huancharuna Perales EM, Schieder 0 (1993) Plant regeneration from leaf protoplasts of apple. Plant Cell Tissue Organ Cult 34: 71–76

    Article  Google Scholar 

  • Jungnickel F, Zaid S (1992) Micropropagation of African violets (Saintpaulia sp. and cvs.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 20. High-Tech and Micro-propagation IV. Springer, Berlin Heidelberg New York, pp 357–395

    Google Scholar 

  • Kao KN, Michayluk MR (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126: 105–110

    Article  CAS  Google Scholar 

  • Lê-Công-Linh (1987) Studies on isolation of petiole protoplasts from Saintpaulia ionantha Wendl., cv. Vroni. In: Collet GF (ed) COST 87. Report of Symp on the Micropropagation of Saintpaulia. Lullier, Switzerland, Commission of the European Communities, pp 69–71

    Google Scholar 

  • Moore HE (1957) African violets, gloxinias and their relatives; a guide to the cultivated generiads. Macmillan, New York

    Google Scholar 

  • Orczyk W, Malepszy S (1985) In vitro culture of Cucumis sativus L. — V. Stabilizing effect of glycine on leaf protoplasts. Plant Cell Rep 4: 269–273

    Google Scholar 

  • Schnabl H, Youngman RJ, Zimmermann U (1983) Maintenance of plant cell membrane integrity and function by the immobilisation of protoplasts in alginate. Planta 158: 392–397

    Article  CAS  Google Scholar 

  • Sree Ramulu K, Dijkhuis P, Roest S (1983) Phenotypic variation and ploidy level of plants regenerated from protoplasts of tetraploid potato (Solanum tuberosum L. cv. Bintje ). Theor Appl Genet 65: 329–338

    Google Scholar 

  • Wendland H (1893) Saintpaulia ionantha. Gartenflora 42: 320–324

    Google Scholar 

  • Widholm JM (1972) The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol 47 (4): 189–194

    PubMed  CAS  Google Scholar 

  • Winkelmann T, Grunewaldt T (1992) Plant regeneration from protoplasts of Saintpaulia ionantha H. Wendl. Gartenbauwissenschaft 57 (6): 284–287

    Google Scholar 

  • Winkelmann T, Grunewaldt J (1993) Plant regeneration from protoplasts of Saintpaulia ionantha H. Wendl., In: Schiva T, Mercuri A (eds) Creating genetic variation in ornamentals. Proc XVIIth EUCARPIA Symp Sect Ornamentals, Sanremo, 1. —5.3.1993, Institut Sperimentale per la Floricultura, Sanremo, pp 117–125

    Google Scholar 

  • Windelmann T, Grunewaldt J (1995a) Analysis of protoplast-derived plants of Saintpaulia ionantha H. Wendl. Plant Breed 114: 346–350

    Google Scholar 

  • Windelmann T, Grunewaldt J (1995b) Genotypic variability for protoplast regeneration in Saintpaulia ionantha H. Wendl. Plant Cell Rep 14: 707–707

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1996 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Winkelmann, T., Grunewaldt, J. (1996). Regeneration of Plants from Protoplasts of Saintpaulia ionantha H. Wendl. (African Violet). In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering VII. Biotechnology in Agriculture and Forestry, vol 38. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-09368-9_13

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-09368-9_13

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08240-5

  • Online ISBN: 978-3-662-09368-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics