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Genome Sequencing

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The Lotus japonicus Genome

Part of the book series: Compendium of Plant Genomes ((CPG))

Abstract

The current Lotus japonicus reference genome sequence is based on a hybrid assembly of Sanger TAC/BAC, Sanger shotgun and Illumina shotgun sequencing data generated from the Miyakojima-MG20 accession. It covers nearly all expressed L. japonicus genes and has been annotated mainly based on transcriptional evidence. Analysis of repetitive sequences suggests that they are underrepresented in the reference assembly, reflecting an enrichment of gene-rich regions in the current assembly. Characterization of Lotus natural variation by resequencing of L. japonicus accessions and diploid Lotus species is currently ongoing, facilitated by the MG20 reference sequence.

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Author information

Authors and Affiliations

  1. Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-Ku, Sendai, 980-8577, Japan

    Shusei Sato

  2. Centre for Carbohydrate Recognition and Signalling, Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10, 8000, Aarhus C, Denmark

    Stig U. Andersen

Authors
  1. Shusei Sato
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  2. Stig U. Andersen
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Corresponding author

Correspondence to Shusei Sato .

Editor information

Editors and Affiliations

  1. Kazusa DNA Research Institute, Kisarazu, Chiba, Japan

    Satoshi Tabata

  2. Department of Molecular Biology and Genetics, University of Aarhus, Aarhus, Denmark

    Jens Stougaard

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© 2014 Springer-Verlag Berlin Heidelberg

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Sato, S., Andersen, S.U. (2014). Genome Sequencing. In: Tabata, S., Stougaard, J. (eds) The Lotus japonicus Genome. Compendium of Plant Genomes. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-44270-8_4

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