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Structure and function of organellar ribosomes as revealed by cryo-EM

  • Chapter
Ribosomes

Abstract

During the last decade ground-breaking progress was made in resolving the structure of ribosomes from several bacterial and archaeal species. A number of high-resolution X-ray crystallographic structures of bacterial ribosomes, complexes, and cryo-electron microscopic (cryo-EM) structures of both prokaryotic and eukaryotic cyto-plasmic ribosomes in various functional states were resolved. More recently, the first X-ray crystallographic structures of eukaryotic ribosomes, the yeast 80S ribosome (Ben-Shem et al. ,2010; also see chapter by Jenner and coworkers in this volume)and a protozoan 40S ribosomal subunit in complex with eukaryotic initiation factor 1 (Rabl et al., 2011)at ~4 Å resolution, have been obtained.Those studies have helped us tremendously in understanding some of the key functions of cytoplasmic ribosomes.Certain organelles of the cell, such as mitochondria and chloroplasts, have their own translational machineries,including ribosomes (Harris et al., 1994; O’Brien, 2002), for the synthesis of proteins that are involved primarily in oxidative phosphorylation and photosynthesis, respectively. Structural studies of organellar ribosomes have lagged behind studies of cyto plasmic ribosomes. In this article, we describe the current state of the structural information available for the organellar ribo somes, which was obtained by using the techniques of single-particle cryo-EM and three-dimensional image processing in our laboratory.

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Authors
  1. Rajendra K. Agrawal
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  2. Manjuli R. Sharma
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  3. Aymen Yassin
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  4. Indrajit Lahiri
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  5. inda L. Spremulli
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Editors and Affiliations

  1. Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry, 37077, Göttingen, Germany

    Marina V. Rodnina  & Wolfgang Wintermeyer  & 

  2. Howard Hughes Medical Institute Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA

    Rachel Green

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Agrawal, R.K., Sharma, M.R., Yassin, A., Lahiri, I., Spremulli, i.L. (2011). Structure and function of organellar ribosomes as revealed by cryo-EM. In: Rodnina, M.V., Wintermeyer, W., Green, R. (eds) Ribosomes. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0215-2_8

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