Abstract
Background: Hyperbaric oxygenation is a readily available treatment modality, and its ability to improve neurological outcomes in a variety of animal models has been demonstrated. This study was designed to investigate the use of a single pretreatment regimen of either hyperbaric oxygenation or normobaric oxygenation to determine its effects in a murine model of surgically induced brain injury (SBI). Materials and Methods: Hyperbaric oxygen (2.5ATM, 1 h), normobaric oxygen (100% FIO2, 1 h) or room air (21% FIO2, 1 h) was applied on CD-1 mice immediately, or at 1, 2 or 3 h followed by SBI or sham surgical operation. Neurological assessment of the animals was done by a blinded observer at 24 and 72 h using a 21-point modified Garcia scale, wire hanging test, and beam balance test. The brain edema was evaluated using brain water content at 24 and 72 h after SBI. Results: There was no statistically significant difference in the mortality rate after treatment compared with the SBI group. The brain water content after SBI was significantly increased in the right (ipsilateral) frontal lobe surrounding the site of surgical resection compared with the sham group. Both hyperbaric and normobaric oxygen treatment significantly increased the brain edema and worsened the neurological outcomes using a 21-point Garcia score compared with the SBI group. The brain edema at 24 h after injury was most pronounced in the group treated with normobaric oxygenation 2 h prior to surgery. These differences disappeared at 72 h after SBI. Conclusion: Immediate pretreatment with either hyperbaric (2.5ATM, 1 h) or normobaric oxygen (100% FIO2, 1 h) increased brain edema and worsened neurological function at 24 h following SBI.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Rockswold GL, Ford SE (1985) Preliminary results of a prospective randomized trial for treatment of severely brain-injured patients with hyperbaric oxygen. Minn Med 68:533–535
Rockswold SB, Rockswold GL, Vargo JM, Erickson CA, Sutton RL, Bergman TA, Biros MH (2001) Effects of hyperbaric oxygenation therapy on cerebral metabolism and intracranial pressure in severely brain injured patients. J Neurosurg 94:403–411
Daugherty WP, Levasseur JE, Sun D, Rockswold GL, Bullock MR (2004) Effects of hyperbaric oxygen therapy on cerebral oxygenation and mitochondrial function following moderate lateral fluid-percussion injury in rats. J Neurosurg 101:499–504
Mink RB, Dutka AJ (1995) Hyperbaric oxygen after global cerebral ischemia in rabbits does not promote brain lipid peroxidation. Crit Care Med 23:1398–1404
Rockswold SB, Rockswold GL, Defillo A (2007) Hyperbaric oxygen in traumatic brain injury. Neurol Res 29:162–172
Veltkamp R, Siebing DA, Sun L, Heiland S, Bieber K, Marti HH, Nagel S, Schwab S, Schwaninger M (2005) Hyperbaric oxygen reduces blood-brain barrier damage and edema after transient focal cerebral ischemia. Stroke 36:1679–1683
Calvert JW, Cahill J, Zhang JH (2007) Hyperbaric oxygen and cerebral physiology. Neurol Res 29:132–141
Ren H, Wang W, Ge Z, Zhang J (2001) Clinical, brain electric earth map, endothelin and transcranial ultrasonic Doppler findings after hyperbaric oxygen treatment for severe brain injury. Chin Med J (Engl) 114:387–390
Buras JA, Reenstra WR (2007) Endothelial-neutrophil interactions during ischemia and reperfusion injury: basic mechanisms of hyperbaric oxygen. Neurol Res 29:127–131
Vlodavsky E, Palzur E, Soustiel JF (2006) Hyperbaric oxygen therapy reduces neuroinflammation and expression of matrix metalloproteinase-9 in the rat model of traumatic brain injury. Neuropathol Appl Neurobiol 32:40–50
Palzur E, Zaaroor M, Vlodavsky E, Milman F, Soustiel JF (2008) Neuroprotective effect of hyperbaric oxygen therapy in brain injury is mediated by preservation of mitochondrial membrane properties. Brain Res 1221:126–133
Ostrowski RP, Colohan AR, Zhang JH (2005) Mechanisms of hyperbaric oxygen-induced neuroprotection in a rat model of subarachnoid hemorrhage. J Cereb Blood Flow Metab 25:554–571
Peng Z, Ren P, Kang Z, Du J, Lian Q, Liu Y, Zhang JH, Sun X (2008) Up-regulated HIF-1alpha is involved in the hypoxic tolerance induced by hyperbaric oxygen preconditioning. Brain Res 1212:71–78
Liu Z, Jiao QF, You C, Che YJ, Su FZ (2006) Effect of hyperbaric oxygen on cytochrome C, Bcl-2 and Bax expression after experimental traumatic brain injury in rats. Chin J Traumatol 9:168–174
Vlodavsky E, Palzur E, Feinsod M, Soustiel JF (2005) Evaluation of the apoptosis-related proteins of the BCL-2 family in the traumatic penumbra area of the rat model of cerebral contusion, treated by hyperbaric oxygen therapy: a quantitative immunohistochemical study. Acta Neuropathol 110:120–126
Nemoto EM, Betterman K (2007) Basic physiology of hyperbaric oxygen in brain. Neurol Res 29:116–126
Niklas A, Brock D, Schober R, Schulz A, Schneider D (2004) Continuous measurements of cerebral tissue oxygen pressure during hyperbaric oxygenation–HBO effects on brain edema and necrosis after severe brain trauma in rabbits. J Neurol Sci 219:77–82
Veltkamp R, Sun L, Herrmann O, Wolferts G, Hagmann S, Siebing DA, Marti HH, Veltkamp C, Schwaninger M (2006) Oxygen therapy in permanent brain ischemia: potential and limitations. Brain Res 1107:185–191
Lee S, Jadhav V, Ayer RE, Rojas H, Hyong A, Lekic T, Tang J, Zhang JH (2009) Dual effects of melatonin on oxidative stress after surgical brain injury in rats. J Pineal Res 46(1):43–48
Demchenko IT, Welty-Wolf KE, Allen BW, Piantadosi CA (2007) Similar but not the same: normobaric and hyperbaric pulmonary oxygen toxicity, the role of nitric oxide. Am J Physiol Lung Cell Mol Physiol 293:L229–L238
Nonaka Y, Shimazawa M, Yoshimura S, Iwama T, Hara H (2008) Combination effects of normobaric hyperoxia and edaravone on focal cerebral ischemia-induced neuronal damage in mice. Neurosci Lett 441:224–228
Qin Z, Song S, Xi G, Silbergleit R, Keep RF, Hoff JT, Hua Y (2007) Preconditioning with hyperbaric oxygen attenuates brain edema after experimental intracerebral hemorrhage. Neurosurg Focus 22:E13
Demchenko IT, Boso AE, O’Neill TJ, Bennett PB, Piantadosi CA (2000) Nitric oxide and cerebral blood flow responses to hyperbaric oxygen. J Appl Physiol 88(4):1381–1389
Acknowledgement
This study is partially supported by NIH NS053407 to J.H. Zhang.
Conflict of interest statement We declare that we have no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer-Verlag/Wien
About this chapter
Cite this chapter
Westra, D., Chen, W., Tsuchiyama, R., Colohan, A., Zhang, J.H. (2011). Pretreatment with Normobaric and Hyperbaric Oxygenation Worsens Cerebral Edema and Neurologic Outcomes in a Murine Model of Surgically Induced Brain Injury. In: Zhang, J., Colohan, A. (eds) Intracerebral Hemorrhage Research. Acta Neurochirurgica Supplementum, vol 111. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0693-8_41
Download citation
DOI: https://doi.org/10.1007/978-3-7091-0693-8_41
Published:
Publisher Name: Springer, Vienna
Print ISBN: 978-3-7091-0692-1
Online ISBN: 978-3-7091-0693-8
eBook Packages: MedicineMedicine (R0)