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Monoamine oxidase activities in human cystic and colonic arteries — influence of age

  • Conference paper
MAO — The Mother of all Amine Oxidases

Part of the book series: Journal of Neural Transmission. Supplement ((NEURAL SUPPL,volume 52))

Summary

The deamination of 5-hydroxytryptamine, phenylethylamine and benzylamine by monoamine oxidases (MAO-A and B) and semicarbazide sensitive amine oxidase (SSAO) respectively has been studied in homogenates of human cystic and colonic arteries by radiochemical assays. In cystic artery the deamination is mainly carried out by SSAO with a lower participation of MAO-B. The kinetic parameters were: to MAO-B the Vmax = 15.11 ± 0.51nmol/mg protein.h and the Km = 78.51 ± 5.16 μM (±SE) and to SSAO the Vmax = 211.70 ± 8.75nmol/mg protein.h and the Km = 211.51 ± 23.27 μM (±SE). We could not measure MAO-A activity in our experimental conditions and also the levels of catecholamines are very low and the histological studies show a poor innervation in these tissues. In colonic artery the kinetic parameters were: to MAO-B the Vmax = 5.09 ± 0.31nmol/mg protein.h and the Km = 29.12 ± 4.55 μM (±SE) and to SSAO the Vmax = 273.67 ± 8.35nmol/mg protein.h and the Km = 197.89 ± 21.81 μM (±SE). In this artery we could find MAO-A in five among the nine samples studied and the kinetic parameters were: the Vmax = 14.48 ± 0.82nmol/mg protein.h and the Km = 136.40 ± 25.46 μM.

As we have performed the experiments with human vessels from donors with different age we could not find any relationship between the activity or affinity, in MAO-B and SSAO, with age. Nevertheless, the results show in cystic artery an increase in the affinity of MAO-B with age when we consider the female group which suggests a possible role of the hormonal condition in this behaviour.

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Author information

Authors and Affiliations

  1. Laboratório de Farmacologia Faculdade de Farmácia da, Universidade de Coimbra, Largo de D. Dinis, 3030, Coimbra, Portugal

    Dr. I. V. Figueiredo, Dr. I. V. Figueiredo, M. D. Cotrim & M. M. Caramona

  2. Department of Pharmacology, Cambridge University, UK

    A. Martinez Coscolla & B. A. Callingham

Authors
  1. Dr. I. V. Figueiredo
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  2. A. Martinez Coscolla
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  3. M. D. Cotrim
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  4. M. M. Caramona
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  5. B. A. Callingham
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Editor information

Editors and Affiliations

  1. Department of Pharmacology, The Bruce Rappaport Faculty of Medicine, Technion — Israel Institute of Technology, Haifa, Israel

    J. P. M. Finberg  & M. B. H. Youdim  & 

  2. Clinical Neurochemistry, Department of Psychiatry, University of Würzburg, Federal Republic of Germany

    P. Riederer

  3. Biochemistry Department, Trinity College, Dublin, Ireland

    K. F. Tipton

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© 1998 Springer-Verlag Wien

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Figueiredo, I.V., Martinez Coscolla, A., Cotrim, M.D., Caramona, M.M., Callingham, B.A. (1998). Monoamine oxidase activities in human cystic and colonic arteries — influence of age. In: Finberg, J.P.M., Youdim, M.B.H., Riederer, P., Tipton, K.F. (eds) MAO — The Mother of all Amine Oxidases. Journal of Neural Transmission. Supplement, vol 52. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6499-0_20

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  • DOI: https://doi.org/10.1007/978-3-7091-6499-0_20

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-83037-6

  • Online ISBN: 978-3-7091-6499-0

  • eBook Packages: Springer Book Archive

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