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Effects of β2-Agonist Administration on Bacterial Phagocytosis by Splenic Macrophages in Mice

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Physical Activity, Exercise, Sedentary Behavior and Health

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Abstract

We examined the effects of 6-week daily administration of an anabolic dose of a β2-agonist clenbuterol (1.0 mg/kg body weight/day) on the phagocytic capacity of splenic macrophages of 5-week-old male C57BL/6 J mice against Escherichia coli. After 24 h of cessation of clenbuterol or vehicle administration, splenic adherent cells were isolated and analyzed by flow-cytometry. The cells were separated into three subpopulations based on their size. The isolated cells included small cells, which expressed markedly higher levels of macrophage receptor with collagenous structure. Furthermore, these cells exhibited higher phagocytic capacity against E. coli when compared to other subpopulations. The phagocytic capacity of the small cells was clearly suppressed after clenbuterol administration. These results suggest that chronic utilization of clenbuterol as a doping drug impairs bacterial clearance mediated by highly phagocytic splenic macrophages.

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Author notes

  1. Ken Shirato

    Present address: Department of Molecular Predictive Medicine and Sport Science, School of Medicine, Kyorin University, Mitaka, Tokyo, Japan

  2. Shogo Sato

    Present address: University of Texas Southwestern Medical Center, Dallas, TX, USA

Authors and Affiliations

  1. Laboratory of Physiological Sciences, Faculty of Human Sciences, Waseda University, Saitama, Japan

    Ken Shirato, Shogo Sato, Madoka Sato, Yoko Hashizume & Kazuhiko Imaizumi

  2. Administration Office, Joetsu University of Education, Niigata, Japan

    Kaoru Tachiyashiki

  3. Global COE Doctoral Program, Graduate School of Sport Sciences, Waseda University, Saitama, Japan

    Kazuhiko Imaizumi

Authors
  1. Ken Shirato
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  2. Shogo Sato
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  3. Madoka Sato
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  4. Yoko Hashizume
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  5. Kaoru Tachiyashiki
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  6. Kazuhiko Imaizumi
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Corresponding author

Correspondence to Ken Shirato .

Editor information

Editors and Affiliations

  1. Faculty of Sport Sciences, Waseda University, Saitama, Japan

    Kazuyuki Kanosue

  2. Waseda Institute of Sports Nutrition, Waseda University, Saitama, Japan

    Satomi Oshima

  3. School of Kinesiology, Shanghai University of Sport, Shanghai, China

    Zhen-Bo Cao

  4. Faculty of Sport Sciences, Waseda University, Saitama, Japan

    Koichiro Oka

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Shirato, K., Sato, S., Sato, M., Hashizume, Y., Tachiyashiki, K., Imaizumi, K. (2015). Effects of β2-Agonist Administration on Bacterial Phagocytosis by Splenic Macrophages in Mice. In: Kanosue, K., Oshima, S., Cao, ZB., Oka, K. (eds) Physical Activity, Exercise, Sedentary Behavior and Health. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55333-5_17

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  • DOI: https://doi.org/10.1007/978-4-431-55333-5_17

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-55332-8

  • Online ISBN: 978-4-431-55333-5

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