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Tag-Creation Approaches for Highly Efficient Profiling of Interacting Proteins and Domains

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Photoaffinity Labeling for Structural Probing Within Protein

Abstract

Diazirine-based photoaffinity labeling is recognized as one of the most reliable methods for identification of biomolecular interactions because of its excellent chemical and physical properties. To avoid time-consuming steps in the analysis of a tiny amount of labeled product, functionalization of photoprobe should be an essential subject in this method. However, addition of functions often affects affinity of bioactive molecule. In this chapter, multifunctional diazirine-based photocross-linkers and their strategies for rapid target protein profiling of bioactive molecules are described, especially tagging methods after cross-linking including post-labeling using cleavable function, tandem labeling using clickable function, and fluorogenic labeling. Further, a unique target-visualization strategy is presented for facile identification of labeled site within protein using isotope-coded fluorescent tag, which can easily distinguish the target from the enormous range of biomolecules in analytical process using LC-MS/MS. Without any chemical treatments, a coumarin tag is photochemically generated on ligand-interacting surface of protein through structural change from nonfluorescent photocross-linker unit with accompanying cleavage of ligand molecule.

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Acknowledgments

The author is delighted to recognize the many contributions of my collaborators, whose names appear in the references, and especially grateful to Professor Yasumaru Hatanaka for his pioneering works on multifunctional diazirines. His far-sighted ideas and superior insights have been impressed many researchers in this field. The author is grateful to JSPS KAKENHI (the Grants-in-Aids for Scientific Research from the Japan Society for the Promotion of Sciences) for financial support.

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  1. Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan

    Takenori Tomohiro

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  1. Takenori Tomohiro
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Correspondence to Takenori Tomohiro .

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Editors and Affiliations

  1. University of Toyama, Toyama, Japan

    Yasumaru Hatanaka

  2. Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan

    Makoto Hashimoto

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Tomohiro, T. (2017). Tag-Creation Approaches for Highly Efficient Profiling of Interacting Proteins and Domains. In: Hatanaka, Y., Hashimoto, M. (eds) Photoaffinity Labeling for Structural Probing Within Protein. Springer, Tokyo. https://doi.org/10.1007/978-4-431-56569-7_2

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