Summary
The objective of this study was to investigate the effects of adenovirus vector (Ax-)mediated gene transduction of E. coli β-galactosidase (LacZ) and transforming growth factor-β1 (TGF-β1) into a human chondrocyte-like cell line (HCS-2/8). The expression of transduced genes and their expression periods were examined by 5-bromo-4-chloroindolyl-β-D-galactoside (X-gal) staining, Northern blotting, ELISA, and Western blotting. To assess the influence of TGF-β1 gene transduction, the expression of mRNAs of type II collagen, proteoglycan core protein, matrix metalloproteinase-3 (MMP-3), and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) were examined by Northern blotting. Staining with X-gal indicated that the genes were transduced into 99% of the cells. Expression of the transduced genes in the cells was continued for at least 21 days. Transduction of the TGF-β1 gene enhanced mRNA expressions of type II collagen and proteoglycan core protein, but suppressed MMP-3 mRNA expression in the cells. These results indicate Ax is useful in chondrocyte gene therapy, and it could be an efficient mediator of TGF-β1 gene transduction.
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© 1999 Springer-Verlag Tokyo
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Kubo, T., Arai, Y., Kobayashi, K., Imanishi, J., Takigawa, M., Hirasawa, Y. (1999). Gene Delivery to Chondrocytes Using Adenovirus Vector. In: Tanaka, S., Hamanishi, C. (eds) Advances in Osteoarthritis. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68497-8_8
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DOI: https://doi.org/10.1007/978-4-431-68497-8_8
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