Abstract
Oxidized low-density lipoprotein (OxLDL) is one of the major factors involved in development of atherosclerosis. A close correlation exists between OxLDL levels in human circulating plasma and occurrence of cardiovascular diseases. To see insight into the patho-physiological feature of diseases, gene engineered animal models, such as apolipoprotein-E knockout mouse, are very powerful tool. We developed an ELISA procedure to measure OxLDL present in mouse circulating plasma, by modification of the previously established method. This method would be a useful tool to study the behavior and function of OxLDL in early development of atherosclerosis.
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Ehara S, Ueda M, Naruko T, Haze K, Itoh A, Otuska M, Komatsu R, Matsuo T, Itabe H, Takano T, Tsukamoto Y, Yoshiyama M, Takeuchi K, Yoshikawa J, Becker AE. (2001) Elevated levels of oxidized low density lipoprotein show a positive relationship with the severity of acute coronary syndromes. Circulation. 103: 1955–1960.
Itabe H, Takeshima E, Iwasaki H, Kimura J, Yoshida Y, Imanaka T, Takano T. (1994) A monoclonal antibody against oxidized lipoprotein recognizes foam cells in atherosclerotic lesions. Complex formation of oxidized phosphatidylcholine and polypeptides. J. Biol Chem. 269: 15274–15279.
Itabe H, Yamamoto H, Imanaka T, Shimamura K, Uchiyama H, Kimura J, Sanaka T, Hata Y, Takano T. (1996) Sensitive detection of oxidatively modified low density lipoprotein using a monoclonal antibody. J. Lipid Res. 37: 45–53.
Itabe H, Ueda M. (2007) Measurement of plasma oxidized low-density lipoprotein and its clinical implications. J. Atheroscler. Thromb. 14: 1–11.
Zhang SH, Reddick RL, Piedrahita JA, Maeda N. (1992) Spontaneous hypercholesterolemia and arterial lesions in mice lacking apolipoprotein E. Science. 258: 468–471.
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Kato, R. et al. (2008). Quantification of mouse oxidized low-density lipoprotein by sandwich ELISA. In: Miyazaki, A., Imawari, M. (eds) New Frontiers in Lifestyle-Related Diseases. Springer, Tokyo. https://doi.org/10.1007/978-4-431-76428-1_16
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DOI: https://doi.org/10.1007/978-4-431-76428-1_16
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-76427-4
Online ISBN: 978-4-431-76428-1
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