Abstract
Most glycoproteins are attached to some of diverse oligosaccharides differing in their monosaccharide compositions, sequences, branches, linkages, and modifications. Liquid chromatography/mass spectrometry (LC/MS) is recognized as a powerful tool for the separation and characterization of these oligosaccharides (Morelle et al. 2006; Zaia 2004). Here, we present the procedure for the LC/MS of N-linked oligosaccharides released from a glycoprotein.
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References
Itoh S, Kawasaki N, Hashii N, Harazono A, Matsuishi Y, Hayakawa T, Kawanishi T (2006) N-linked oligosaccharide analysis of rat brain Thy-1 by liquid chromatography with graphitized carbon column/ion trap-Fourier transform ion cyclotron resonance mass spectrometry in positive and negative ion modes. J Chromatogr A 1103:296–306
Morelle W, Canis K, Chirat F, Faid V, Michalski JC (2006) The use of mass spectrometry for the proteomic analysis of glycosylation. Proteomics 6:3993–4015
Zaia J (2004) Mass spectrometry of oligosaccharides. Mass Spectrom Rev 23:161–227
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Kawasaki, N., Itoh, S., Yamaguchi, T. (2008). LC/MS of N-Linked Oligosaccharides. In: Taniguchi, N., Suzuki, A., Ito, Y., Narimatsu, H., Kawasaki, T., Hase, S. (eds) Experimental Glycoscience. Springer, Tokyo. https://doi.org/10.1007/978-4-431-77924-7_11
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DOI: https://doi.org/10.1007/978-4-431-77924-7_11
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-77923-0
Online ISBN: 978-4-431-77924-7
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