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Reconstructing the Repertoire of Mite Allergens by Recombinant DNA Technology

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Allergy Frontiers: Future Perspectives

Part of the book series: Allergy Frontiers ((ALLERGY,volume 6))

Abstract

House dust mites are a major source of inhalant allergens worldwide. More than 20 groups of house dust mite allergens have been officially recognized and listed in the World Health Organization – International Union of Immunological Societies (WHO/IUIS) list of allergens, (www.allergen.org). Our recent studies however show more than 40 groups may be present in the various species of dust mites with allergenic specificities varying from one to the other. One key feature of what is observed from the identification of these allergens is the large redundancy of what we can call an “allergen” across different species of mites as well as across diverse organisms. In this article, we also discuss the role and limitations of recombinant allergens particularly the expression systems used to produce these allergens and the difficulty in re-folding selected number of these proteins appropriately. Nevertheless, recombinant allergens have been essential in our understanding of the primary structures of these antigens, their epitopes and immunological properties.

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Authors and Affiliations

  1. Department of Biological Sciences, National University of Singapore, Allergy and Molecular Immunology Laboratory, Lee Hiok Kwee Functional Genomics Laboratories, 14, Science Drive 4, 117543, Singapore

    Seow Theng Ong & Fook Tim Chew

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  1. Seow Theng Ong
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  2. Fook Tim Chew
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Editors and Affiliations

  1. Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, Japan

    Ruby Pawankar

  2. University of Southampton Southampton General Hospital, Tremona Road, Southampton, UK

    Stephen T. Holgate

  3. Children's Mercy Hospitals and Clinics UMKC School of Medicine, 2401 Gillham Road, Kansas City, MO, 64108, USA

    Lanny J. Rosenwasser

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Ong, S.T., Chew, F.T. (2010). Reconstructing the Repertoire of Mite Allergens by Recombinant DNA Technology. In: Pawankar, R., Holgate, S.T., Rosenwasser, L.J. (eds) Allergy Frontiers: Future Perspectives. Allergy Frontiers, vol 6. Springer, Tokyo. https://doi.org/10.1007/978-4-431-99365-0_4

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  • DOI: https://doi.org/10.1007/978-4-431-99365-0_4

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-99364-3

  • Online ISBN: 978-4-431-99365-0

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