Abstract
The establishment of improved technologies for imaging of the pancreas is a key element in addressing several aspects of diabetes pathogenesis. In this respect, the development of a protocol that allows for non-invasive scoring of human islets, or islet β-cells, is of particular importance. The development of such a technology would have profound impact on both clinical and experimental medicine, ranging from early diagnosis of diabetes to the evaluation of therapeutic regimes. Another important task is the development of modalities for high-resolution imaging of experimental animal models for diabetes. Rodent models for diabetes research have for decades been instrumental to the diabetes research community. The ability to image, and to accurately quantify, key players of diabetogenic processes with molecular specificity will be of great importance for elucidating mechanistic aspects of the disease. This chapter aims to overview current progress within these research areas.
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Abbreviations
- BCM:
-
β-cell mass
- BLI:
-
Bioluminescence imaging
- BLT:
-
Bioluminescence tomography
- CLSM:
-
Confocal laser scanning microscopy
- CT:
-
Computed tomography
- LSM:
-
Laser scanning microscopy
- MBT:
-
Multispectral bioluminescence tomography
- MPLSM:
-
Multiphoton laser scanning microscopy
- MRI:
-
Magnetic resonance imaging
- NMRI:
-
Nuclear magnetic resonance imaging
- OCT:
-
Optical coherence tomography
- OPT:
-
Optical projection tomography
- PET:
-
Positron emission tomography
- SPECT:
-
Single photon emission computed tomography
- SPIM:
-
Selective plane illumination microscopy
- SPIO:
-
Superparamagnetic iron oxide
- T1D:
-
Type 1 diabetes
- T2D:
-
Type 2 diabetes
- TPLSM:
-
Two photon laser scanning microscopy
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Acknowledgements
The authors are grateful to Dr. J. Gilthorpe and Dr. J. Sharpe for valuable comments on the manuscript.
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Ahlgren, U., Gotthardt, M. (2010). Approaches for Imaging Islets: Recent Advances and Future Prospects. In: Islam, M. (eds) The Islets of Langerhans. Advances in Experimental Medicine and Biology, vol 654. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3271-3_3
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