Abstract
The induction of apoptosis by influenza virus infection has been shown in vitro and in vivo. Here, we present a quantitative investigation of apoptosis occurring during influenza A vaccine production in Madin-Darby canine kidney microcarrier culture. Flow cytometry was employed for single-cell based analysis of infection, apoptosis and intracellular accumulation of viral nucleoprotein. Apoptotic DNA strand breaks were observed especially during late influenza A virus infection phase. Apoptosis was mainly detected in infected cells, only a small fraction of uninfected cells was apoptotic.
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Schulze-Horsel, J., Schulze, M., Genzel, Y., Reichl, U. (2010). Single-Cell Approach in Influenza Vaccine Production: Apoptosis and Virus Protein Production. In: Noll, T. (eds) Cells and Culture. ESACT Proceedings, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3419-9_5
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DOI: https://doi.org/10.1007/978-90-481-3419-9_5
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