Abstract
Francisella tularensis harbors genes with similarity to genes encoding components of a type VI secretion system (T6SS). These include iglA and iglB, the homologues of which are conserved in T6SSs. They are part of the igl operon, also encompassing the iglC and iglD genes. We have used a yeast two-hybrid system to study the interaction of the Igl proteins of F. tularensis LVS. Previously, we identified a region of IglA necessary for efficient binding to IglB as well as for IglAB protein stability and intra-macrophage growth with an essential role for a conserved α-helical region. Thus, IglA-IglB complex formation is clearly crucial for Francisella pathogenicity and the same interaction is conserved in other human pathogens. Herein, the interaction of IglC with other members of the operon was investigated. It showed no binding to the other members in the yeast two-hybrid assay and we found also that two cysteine residues, C191 and C192, predicted to be putative prenylation sites, played no role for the important contribution of IglC to the intracellular replication of F. tularensis although C191 was important for the stability of the protein.
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Acknowledgements
This work was supported by grants 2006–3426 (JB) and 2006–2877 (AS) from the Swedish Research Council and a grant from the Medical Faculty, Umeå University, and the Umeå Biotech Incubator, Umeå, Sweden. The work was performed in part at the Umeå Centre for Microbial Research (UCMR).
Index words: Francisella, type VI secretion system, Igl proteins, yeast two-hybrid system, IglC, cysteine residues.
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Bröms, J.E., Lavander, M., Sjöstedt, A. (2010). Dissection of the Functions of the IglC Protein of Francisella tularensis . In: Shafferman, A., Ordentlich, A., Velan, B. (eds) The Challenge of Highly Pathogenic Microorganisms. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9054-6_7
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DOI: https://doi.org/10.1007/978-90-481-9054-6_7
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