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Search for Low-Molecular-Weight Biomarkers in Plant Tissues and Seeds Using Metabolomics: Tools, Strategies, and Applications

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Seed Development: OMICS Technologies toward Improvement of Seed Quality and Crop Yield

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Abstract

This chapter summarises the metabolomic strategies currently in force used in plant science and describes the methods used. The metabolite profiling and fingerprinting of plant tissues through MS- and/or NMR-based approaches and the subsequent identification of biomarkers is detailed. Strategies for the microisolation and de novo identification of unknown biomarkers are also discussed. The various approaches are illustrated by a metabolomic study of the maize response to herbivory. A review of recent metabolomic studies performed on seed and crop plant tissues involving various analytical strategies is provided.

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Acknowledgments

Financial support was provided by the Swiss National Science Foundation (Grant no. 205320_135190/1 to J-L.W.). The work was also supported by the National Centre of Competence in Research (NCCR) Plant Survival, a research program of the Swiss National Science Foundation, as well as by an IPP project from the SystemsX.ch initiative.

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Authors and Affiliations

  1. School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, Quai Ernest-Ansermet 30, 1211, Geneva, Switzerland

    Guillaume Marti, Serge Rudaz & Jean-Luc Wolfender

  2. Max Planck Institute for Chemical Ecology, Root-Herbivore Interactions Group, Hans-Knoell-Str., 807745, Jena, Germany

    Matthias Erb

  3. Laboratory for Fundamental and Applied Research in Chemical Ecology, University of Neuchâtel, 2000, Neuchâtel, Switzerland

    Ted Turlings

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  1. Guillaume Marti
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  2. Matthias Erb
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  3. Serge Rudaz
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  4. Ted Turlings
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  5. Jean-Luc Wolfender
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Correspondence to Jean-Luc Wolfender .

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  1. RLABB, Sanepa, Lalitpur, Nepal

    Ganesh K. Agrawal

  2. , Biology, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, 305-8572, Japan

    Randeep Rakwal

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Marti, G., Erb, M., Rudaz, S., Turlings, T., Wolfender, JL. (2012). Search for Low-Molecular-Weight Biomarkers in Plant Tissues and Seeds Using Metabolomics: Tools, Strategies, and Applications. In: Agrawal, G., Rakwal, R. (eds) Seed Development: OMICS Technologies toward Improvement of Seed Quality and Crop Yield. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4749-4_16

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