Abstract
The field ecology of Penicillium verrucosum (important Ochratoxin A producing storage fungus) is poorly understood. It is for example not known whether the fungus can survive, proliferate, and infect cereal plants in the field. For such studies, a sensitive method is needed to screen the presence of the fungus in naturally and artificially infested soils. Prior to experiments with deliberate release of P. verrucosum into the field environment, preliminary experiments were performed regarding four different media (V8, YES, DRYES, and DYSG). Only DYSG has selective and indicative properties that makes it suitable as a medium for recover and easy detection of P. verrucosum from a population of deuteromycete soil fungi. The recovery and detection level of P. verrucosum on DYSG was determined on Petri dishes, plated with mixtures from a suspension of a non-spiked soil and decreasing amounts of a suspension from a soil, spiked with conidia of P. verrucosum. Results showed a very high recovery (91% and 112%) of P. verrucosum and a detection level of 300 P. verrucosum CFU/g dry soil. In conclusion: given a good selective medium, classical microbiological methods may well be used for monitoring fungi deliberately released into the environment.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Breitholz-Emanuelson, A., Olsen, M., Oskarsson, A., and Hult, K. (1993). Ochratoxin A in Cow’s Milk and in Human Milk with Corresponding Human Blood Samples, J. AOAC Int. 76, 842–846.
Büchmann, N.B. and Hald, B. (1985). Analysis, occurrence and control of Ochratoxin A residues in Danish pig kidneys, Food Additives and Contaminants 2, 193–199.
Frisvad, J.C. (1983). A selective and indicative medium for groups of Penicillium viridicatum producing different mycotoxins in cereals, Journal of Applied Bacteriology 54, 409–416.
Frisvad, J.C., Thrane, U., and Filtenborg, O. (1986). Comparison of five media for enumeration of mesophilic mycoflora of foods, in A.D. King, J.I. Pitt, L.R. Beuchat, and J.E.L. Corry (eds.), Methods for the mycological examination of food, Plenum Press, New York, pp. 138–142.
Frisvad, J.C. and Filtenborg, O. (1989) Terverticillate Penicillia: Chemotaxonomyand Mycotoxinproduction, Mycologia 81, 837–861.
Frisvad, J.C., Filtenborg, O., Lund, F., and Thrane, U. (1992) New Selective Media for the Detection of Toxigenic Fungi in Cereal Products, Meat and Cheese, in R.A. Samson, Hocking, A.D., Pitt, J.I., and King, A.D. (eds.), Modern Methods in Food Mycology, Elsevier, Amsterdam, pp. 275–285.
Gams, W., van der Aa, H.A., van der Plaats-Niterink, A.J., Samson, R.A., and Stalpers, J.A. (1987) CBS Course of Mycology, CBS, Baarn.
Hult, K., Plestina, R., Habazin-Novak, V., Radic, B., and Ceovic, S. (1982) Ochratoxin A in Human Blood and Balkan Endemic Nephropathy, Arch. Toxicol. 51, 313–321.
Kornerup, A. og Wanscher, J.H. (1969) Farver i farver, Politikens forlag, København.
Lund, F., Thrane, U., Frisvad, J.C., and Filtenborg, O. (1992) IBT List of fungal cultures and their secondary metabolites, 1st edition, Dept. of Biotechnology, The Technical University of Denmark.
Madhyastha, S., Marquardt, R.R., and Abramson, D. (1993) Effect of ochratoxin producing fungi on the chemical composition of wheat and barley, Journal of Food Quality 16, 287–299.
Pitt, J.I. (1979) The Genus Penicillium and its Teleomorphic States Eupenicillium and Talaromyces, Academic Press, New York.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Kluwer Academic Publishers
About this chapter
Cite this chapter
Elmholt, S., Hestbjerg, H. (1996). Recovery and Detection of Deuteromycete Conidia from Soil. In: Jensen, D.F., Jansson, HB., Tronsmo, A. (eds) Monitoring Antagonistic Fungi Deliberately Released into the Environment. Developments in Plant Pathology, vol 8. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1698-2_7
Download citation
DOI: https://doi.org/10.1007/978-94-009-1698-2_7
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7260-1
Online ISBN: 978-94-009-1698-2
eBook Packages: Springer Book Archive