Summary
This is a method for determination of lignin and humic substances in waters by HPLC with fluorimetric detection. Water samples are extracted with ethyl acetate and the carboxylic acids in these extracts are labeled with 4-Br-MMC. Results indicate that this method might be applicable to characterzation of humic waters and differentiation of humic and lignin compounds in recipient waters of pulp mills. Further experiments are needed.
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References
KNUUTINEN, J., VIRKKI, L., PASTINEN, O. and PAASIVIRTA, J. (1986) Aqueous liquid chromatographic determination of humic and lignin compounds in surface waters. International Humic Substances Society Third International Meeting, August 4–8, 1986 in Oslo Norway; Abstract p. 102.
KNUUTINEN, J., VIRKKI, L., MANNILA, P., MIKKELSON, P. and PAASIVIRTA, J. (1987) Aqueous high-performance size-exclusion chromatography of dissolved organic matter in natural waters. Water Res. (submitted).
LAM, S. and GRUSHKA, E. (1978) Labelling of fatty acids with 4-bromo-methyl-7-methoxy-coumarin via crown ether catalyst for fluorimetric detection in high-performance liquid chromatography. J. Chromatogr. 158, 207–214.
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© 1988 ECSC, EEC, EAEC, Brussels and Luxembourg
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Mikkelson, P., Paasivirta, J., Knuutinen, J. (1988). HPLC/Fluorescence Spectrometry in Analyses of Pulp Mill Wastes in Recipients. In: Angeletti, G., Bjørseth, A. (eds) Organic Micropollutants in the Aquatic Environment. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2989-0_12
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DOI: https://doi.org/10.1007/978-94-009-2989-0_12
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7843-6
Online ISBN: 978-94-009-2989-0
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