Skip to main content

Clonal analysis of bone marrow and macrophage cultures

  • Chapter
Mononuclear Phagocytes

Abstract

The identification and organization of the major cell types that make up the mononuclear phagocyte system is based on conventional histological staining patterns. This system is discussed in the chapters by Van Furth (1), Van der Meer (2), Ginsel (3), and Hoefsmit (4). About two decades ago a similar situation existed for the identification of cells of the lymphoid system when only large, medium and small lymphocytes were delineated by histological and morphological characteristics. Today, the lymphoid system has been subdivided into a multitude of functional subpopulations based on surface membrane differences resolved by antibodies.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 699.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Van Furth R, Diesselhoff-den Dulk MMC, Sluiter W, Van Dissel JT: New perspectives on the kinetics of mononuclear phagocytes, 1985. This volume.

    Google Scholar 

  2. Van der Meer JWM, Van de Gevel JS, De Water R, Ginsel LA, Wouters CH, Daems WTh, Van Furth R: Proliferation and differentiation of mononuclear phagocytes in vitro, 1985. This volume.

    Google Scholar 

  3. Ginsel LA, De Water R, Van der Meer JWM, Daems WTh: Heterogeneity of 5’nucleotidase activity of wheat-germ agglutinin binding in mononuclear phagocytes, 1985. This volume.

    Google Scholar 

  4. Hoefsmit ECM, Beelen RHJ: The expression of F4/80 antigen and la on peritoneal macrophages in normal and BCG immunized mice, 1985. This volume.

    Google Scholar 

  5. Walker WS, Hester RB, Gandour DM, Stewart CC: Evidence of a distinct progenitor for the Ia-bearing (la’) murine bone marrow-derived mononuclear phagocyte (MNP). In: Phagocytes. Forster O. Landy M, eds. Academic Press NY 1981, pp 229–231.

    Google Scholar 

  6. Rabellino EM and Metcalf DJ: Receptors for C3 and IgG on macrophage, neutrophil and eosinophil colony cells grown in vitro. J Immunol 1975, 115: 688–692.

    PubMed  CAS  Google Scholar 

  7. van Furth R, Raeburn JA, Van Zwet TL: Characteristics of human mononuclear phagocytes. Blood, 1979, 54: 485–500.

    PubMed  Google Scholar 

  8. Ruco LP, Meltzer MS: Macrophage activation for tumoricidal cytotoxicity: Induction of tumoricidal macrophages by supernatants of PPD-stimulated bacillus calmette-guerinimmune spleen cell cultures. J Immunol 1977, 119: 889–896.

    PubMed  CAS  Google Scholar 

  9. Leonard EJ, Ruco LP, Meltzer MS: Characterization of macrophage activation factor, a lymphokine that causes macrophages to become cytotoxic for tumor cells. Cell Immuno1 1978, 41: 347–357.

    Article  CAS  Google Scholar 

  10. Buchmüller Y, Mauel J: Studies on the mechanisms of macrophage activation. J Exp Med 1979, 150: 359–370.

    Article  PubMed  Google Scholar 

  11. Calamai EG, Beller DI, Unanue ER: Regulation of macrophage populations. IV. Modulation of la Expression in bone marrow-derived macrophages. J Immunol 1982, 128: 1692–1694.

    PubMed  CAS  Google Scholar 

  12. Unkeless JC, Gordon S, Reich E: Secretion of plasminogen activator by stimulated macrophages. J Exp Med 1974, 139: 834–850.

    Article  PubMed  CAS  Google Scholar 

  13. Wahl LM, Wahl SM, Mergenhagen SE, Martin GR: Collagenase production by endotoxin-activated macrophages. Proc Natl Acad Sci 1974, 71: 3598–3601.

    Article  PubMed  CAS  Google Scholar 

  14. Werb Z, Gordon S: Elastase secretion by stimulated macrophages: Characterization and regulation. J Exp Med 1975, 142: 361–377.

    Article  PubMed  CAS  Google Scholar 

  15. Gordon S, Werb Z: Secretion of macrophage neutral proteinase is enhanced by colchicine. Proc Natl Acad Sci USA 1976, 73: 872–876.

    Article  PubMed  CAS  Google Scholar 

  16. Stanley ER, Guilbert LJ, Tushinski RJ, Bartelmez SH: CSF-1-A mononuclear phagocyte lineage-specific hemo-poietic growth factor. J Cell Biochem 1983, 21: 151–159.

    Article  PubMed  CAS  Google Scholar 

  17. Stewart CC: Murine mononuclear phagocytes from bone marrow. In: Methods for Studying Mononuclear Phagocytes. Adam DO, Edelson PJ, Kovan H: Academic Press, Inc, New York 1981, pp. 5–20.

    Google Scholar 

  18. Johnson CR, Kitz D, Little JR: A method for the derivation and continuous propagation of cloned murine bone marrow macrophages. J Immunol Methods 1983, 65: 319–332.

    Article  PubMed  CAS  Google Scholar 

  19. Walker EB, Akporiaye ET, Warner NL, Stewart CC: Characterization of subsets of bone marrow-derived macrophages by flow cytometry analysis. J Leuk Biol 1985, 37: 121–136.

    CAS  Google Scholar 

  20. Bell A, Marek LF, Levinstone DS, Merrill C, Sher S, Young IT, Eden M: Loss of division potential in vitro: Aging or Differentiation? Science 1978, 202: 1158–1163.

    Article  PubMed  CAS  Google Scholar 

  21. Levenson R, Housman D: Commitment: How do cells make the decision to differentiate? Cell 1981, 25: 5–6.

    Article  PubMed  CAS  Google Scholar 

  22. Holliday RG, Huschtcha LI, Kerkwood TBL: Cellular aging: Further evidence for the commitment theory. Science 1981, 213: 1505–1508.

    Article  PubMed  CAS  Google Scholar 

  23. Goud ThJLM, van Furth R: Proliferative characteristics of monoblasts grown in vitro. J Exp Med 1975, 142: 1200–1217.

    Article  PubMed  CAS  Google Scholar 

  24. Van der Meer JWM, van de Gevel JS, van Furth R: Characteristics of long-term cultures of proliferating mononuclear phagocytes from bone marrow. J Reticuloendothel Soc 1983, 34: 203–225.

    PubMed  Google Scholar 

  25. Van Furth R, van der Meer JWM, Toivonen II, Rytömaa T: Kenetic analysis of the growth of bone marrow mononuclear phagocytes in long-term cultures. J Reticuloendothel Soc 1983, 34: 227–234.

    PubMed  Google Scholar 

  26. Sanchez-Madrid F, Nagy JA, Robins E, Simon P. Springer TA: A human leukocyte differentiation antigen family with distinct a-subunits and a common b-subunit: The lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/MAC1) and the p150,95 molecule. J Exp Med 1983, 158: 1785.

    Article  PubMed  CAS  Google Scholar 

  27. Ho MK, Springer TA: MAC-2, a novel 32000 Mr mouse macrophage subpopulation-specific antigen defined by monoclonal antibodies. J Immunol 1982, 128: 1221–1228.

    PubMed  CAS  Google Scholar 

  28. Ho MK, Springer TA: Tissue distribution, structural characterization and biosynthesis of MAC-3, a macrophage surface glycoprotein exhibiting molecular weight heterogeneity. J Biol Chem 1983, 258: 636–642.

    PubMed  CAS  Google Scholar 

  29. Ledbetter JA, Herzenberg LA: Xenogenic monoclonal antibodies to mouse differentiation antigens. Immunol Rev 1979, 47: 63–90.

    Article  PubMed  CAS  Google Scholar 

  30. Lanier LL, Gutman GA, Lewis DE, Griswalt ST. Warner NL: Monoclonal antibodies against rat immunoglobulin k chains. Hybridoma 1982, 1: 125.

    Article  PubMed  CAS  Google Scholar 

  31. Watson JD, Prestridge RL: Interleukin 3 and colony-stimulating factors. Immunol Today 1983, 4: 278–280.

    Article  CAS  Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1985 Martinus Nijhoff Publishers, Dordrecht

About this chapter

Cite this chapter

Stewart, C.C., Walker, E.B., Johnson, C., Little, R. (1985). Clonal analysis of bone marrow and macrophage cultures. In: van Furth, R. (eds) Mononuclear Phagocytes. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-5020-7_26

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-5020-7_26

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-8723-0

  • Online ISBN: 978-94-009-5020-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics