Abstract
The use of isolated retinal preparations in a bathing solution is one of the conventional methods for electroretinographic investigation of the retina. The conditions of the experimental procedure deviate to a considerable extent from the physiological conditions of in vivo studies. We have reported a series of studies of the retinal function and metabolism using a perfusion technique of isolated bovine eyes, the “Living Extracorporeal Bovine Eye” (Imaizumi, 1974; Imaizumi et al., 1972, 1973, 1974; Mera, 1972; Otsuko, 1973; Takahashi, 1974, Tazawa & Seaman, 1972), which is able to maintain the eyes at a nearly physiological state, developed by one of the present authors (Tazawa) in collaboration with Seaman (Tazawa) Seaman, 1972).
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References
Brindley, G. B. The sources of slow electrical activity in the frog’s retina. J. Physiol. 140: 247–261 (1968).
Hoff, M. & P. Gouras. Tolerance of ammalian retina to circulatory arrest. pp. 57-63 in: Proceedings Xth ISCERG Symposium, Docum. Ophthal. Proc. Series (1972).
Imaizumi, H. Influence of sodium ion concentration on electroretinogram of the living extracorporeal bovine eye. Acta Soc. Ophthal. Jap. 78: 831–843 (1974).
Imaizumi, K. et al. Electrophysiological application of the living extracorporeal bovine eyes. pp. 119–123 in: Advances in experimental medicine and biology, Vol. 24: The visual system. Neorphysiology, biophysics and their clinical applications. Plenum, New York (1972).
Imaizumi, K. et al. ERG and O2-tension of the retina in the living extracorporeal eye. Fifth Afro-Asian Congr. Ophthal, pp. 420-425 (1972).
Imaizumi, K. et al. Recording the slow components of retinal electric responses of the living extracorporeal bovine eye. Folia Ophthal. Jap. 24: 544–545 (1973).
Imaizumi, K. et al. Effects of [Na+] in the blood on electroretinogram. Folia Ophthal. Jap. 25: 1134–1136 (1974).
Imaizumi, K. et al. Electroretinogram of the living extracorporeal bovine eye and blood flow. Jap. J. Ophthal. 18: 177–186 (1974).
Mera, H. Electroretinographic studies on the living extracorporeal eye perfused with high potassium-and sodium-blood. Acta Soc. Ophthal. Jap. 76: 921–929 (1972).
Otsuko, T. Erythrocytes substitute for perfusion of the living extracorporeal bovine eye. An electroretinographical study. Acta Soc. Ophthal. Jap. 77: 1102–1115 (1973).
Takahashi, Y. The minimum oxygen volume necessary for maintaing ERG of the living extracorporeal bovine eye. Jap. J. Ophthal. 18: 177–186 (1974).
Tazawa, Y. & A. J. Seaman. The electroretinogram of the living extracorporeal bovine eye. The influence of anoxia and hypothermia. Invest. Ophthal. 11: 691–698 (1972).
Tomita, T. Studies in interretinal action potential with low-resistance micro-electrode. J. Neurophysiol. 15: 75–84 (1952).
Yonemura, D. The oscillatory potential of the electroretinogram. Acta Soc. Ophthal. Jap. 66: 1566–1684 (1962).
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© 1978 Dr W. Junk b.v. Publishers
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Tazawa, Y., Imaizumi, H., Mera, H., Otsuka, T., Takahashi, Y., Imaizumi, K. (1978). Changes in ERG Wave form of the Isolated Bovine Eye during Long Term Perfusion. In: François, J., De Rouck, A., Pearlman, J.T., Kelsey, J. (eds) Electrodiagnosis, Toxic Agents and Vision. Documenta Ophthalmologica Proceedings Series, vol 15. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-9957-2_40
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DOI: https://doi.org/10.1007/978-94-009-9957-2_40
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