Abstract
A reporter gene system based on the expression of green fluorecense protein (GFP) was developed for monitoring the dynamics of apoptotic cell death. The expression plasmid was stably introduced into CHO-DHFR− cells in which GFP expression is driven by the apoptosis-responsive c-fos promoter. Single clones were isolated showing lower GFP expression in serum-free medium, but exhibited high-levels of short-term GFP expression in the serum responsive elements (SRE) induced state. Data on the characteristics of this system for monitoring the dynamics of apoptotic cell death induced by growth factor (serum) deprivation and exotoxins from Pseudomonas aeruginosa PAO1 are presented.
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© 2001 Springer Science+Business Media Dordrecht
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Bi, JX., Wirth, M., Beer, C., Sabra, W., Zeng, AP. (2001). The Use of a C-FOS-GFP Reporter System for Monitoring Apoptosis of Animal Cells Induced by Exotoxins of Pseudomonas Aeruginosa . In: Lindner-Olsson, E., Chatzissavidou, N., Lüllau, E. (eds) Animal Cell Technology: From Target to Market. ESACT Proceedings, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0369-8_18
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DOI: https://doi.org/10.1007/978-94-010-0369-8_18
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3897-3
Online ISBN: 978-94-010-0369-8
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