Abstract
Glutamine is an essential amino acid that provides a major energy source in mammalian cells. It plays the role of both carbon and nitrogen sources. The concentration of glutamine strongly influences the cell metabolism that governs cell growth and monoclonal antibody productivity. Many experiments have been carried out in order to understand the relationship between glutamine concentration and cell metabolism (1–3), however it is not so clear. Recently, the technique for calculating metabolic fluxes has been developed. In this study a chemostat culture was studied under a wide range of glutamine feed concentrations and change of its metabolism was examined by utilizing metabolic flux technique.
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© 2001 Springer Science+Business Media Dordrecht
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Matsuoka, H., Andoh, S., Matsuda, Y., Kaneko, H., Takeda, T. (2001). Influences of Glutamine Concentration on Cellular Metabolism in Continuous Culture of Hybridoma. In: Lindner-Olsson, E., Chatzissavidou, N., Lüllau, E. (eds) Animal Cell Technology: From Target to Market. ESACT Proceedings, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0369-8_37
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DOI: https://doi.org/10.1007/978-94-010-0369-8_37
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3897-3
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