Abstract
Rabbit polymorphonuclear leukocytes (PMNs) elicited by intraperitoneal injections of glycogen were prepared and suspended in Eagle’s MEM (Davidson et al. (1978). Br. J. Pharmacol. 63, 407P). PMNs were incubated in the presence and absence of serum opsonized zymosan (1mg/ml) and/or 10-5 mol/1 calcium ionophore (A23187) for 2 h at 37°C. Cells were removed by centrifugationa and aliquots of the supernatant removed for enzyme assays and radio-immunoassay of PGE2. The remaining supernatant was acidified and extracted with diethyl ether. The ether extracts were either used for bioassay of PGE-like activity (rat fundic strip) or derivatized to form the methyl ester, methoxime, tertiary butyldimethylsilyl ethers of the prostaglandins and TXB2 and the levels were measured by quantitative gas chromatography mass spectrometry (GC-MS).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1980 MTP Press Limited
About this chapter
Cite this chapter
Davidson, E.M., Smith, M.J.H., Ford-Hutchinson, A.W. (1980). Release of thromboxane B2 and PGE2 by rabbit polymorphonuclear leukoctyes (Abstract). In: Willoughby, D.A., Giroud, J.P. (eds) Inflammation: Mechanisms and Treatment. Inflammation: Mechanisms and Treatment, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-9423-8_42
Download citation
DOI: https://doi.org/10.1007/978-94-010-9423-8_42
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-9425-2
Online ISBN: 978-94-010-9423-8
eBook Packages: Springer Book Archive