Abstract
Mammalian cells are the preferred production organisms which can produce recombinant proteins in their native, fully glycosylated form. However, the overall cellular productivity is limited, when compared to yeasts and procaryotes. Therefore the main target in bioprocess development for mammalian cell cultures is the enhancement of product yield to achieve economical and competitive product titers. In the outlined presentation we investigated the effects of various compounds on the specific productivity of serum-free growing recombinant CHO cells, with special regard to the effects of the carbohydrate sources. A strictly proportional expressed product formation was observed when compared to the glucose consumption of the corresponding cells. On the other hand, the glutamine metabolism seems to be reduced during enhanced product formation phases. The growth rate was ‘inverse’ regulated depending on the availability of the main carbohydrate (glucose).
The outlined paper describes an ‘easy to perform’ process optimization by controlled addition of glucose/glutamine.
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© 1995 Springer Science+Business Media Dordrecht
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Fieder, J., Schorn, P., Bux, R., Noé, W. (1995). Increase of Productivity in Recombinant Cho-Cells by Enhanced Glucose-Levels. In: Beuvery, E.C., Griffiths, J.B., Zeijlemaker, W.P. (eds) Animal Cell Technology: Developments Towards the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0437-1_26
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DOI: https://doi.org/10.1007/978-94-011-0437-1_26
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4195-9
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