Abstract
Nucleotide sequence analysis of Bradyrhizobium japonicum chromosomal DNA, 9 kb downstream of the hup genes (hydrogenase structural genes), revealed one incomplete and three complete open reading frames. These were designated as hypD’, hypE, hoxX and hox A, respectively, based on the strong homology of the deduced amino acid sequences with those encoded by genes from Azotobacter vinelandii, Escherichia coli, Rhodobacter capsulatus, Rhizobium leguminosarum (hypD’ and hypE) and Alcaligenes eutrophus (hoxX and hoxA). Implication of these findings on the regulation of hydrogenase synthesis by hydrogen, oxygen and nickel in free-living B. japonicum are discussed. Similarly the nucleotide sequence analysis of a 2.2 kb region downstream the B. japonicum hydrogenase structural genes allowed the identification of hupC, hupD and hupF homologous genes forming the distal part of an operon containing the hup structural genes hupS and hupL. A few hundred bp downstream, separated by an AT rich region, an RpoNl/RpoN2 transcribed hupG homologous gene was identified. These ORF–s show significant homology to genes involved in processing, maturation and functional regulation of the hydrogenase complex, previously described in various hydrogen-oxidizing bacteria.
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© 1994 Springer Science+Business Media Dordrecht
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Van Soom, C., Vanderleyden, J., Van Gool, A.P. (1994). Characterization of Bradyrhizobium japonicum chromosomal genes involved in the regulation of hup gene expression in free-living conditions and in controlling hydrogenase activity. In: Graham, P.H., Sadowsky, M.J., Vance, C.P. (eds) Symbiotic Nitrogen Fixation. Developments in Plant and Soil Sciences, vol 57. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1088-4_16
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DOI: https://doi.org/10.1007/978-94-011-1088-4_16
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