Abstract
Among the food microorganisms tested and screened, Bifidobacterium adolescentis showed especially strong mitogenic activity toward murine splenocytes and Peyer’s patch cells, and enhanced the proliferation of ovomucoid-stimulated lymph node cells in an antigen-nonspecific manner. B. adolescentis also enhanced the production of anti-ovomucoid antibody by primed lymph node cells. A lipopolysaccharide inhibitor, polymyxin B, did not inhibit the mitogenic activity of B. adolescentis toward normal BALB/c splenocytes. A high molecular weight (>100 kDa) fraction from the proteinase K-treated cytoplasmic fraction of B. adolescentis, but not a low molecular weight fraction, retained the mitogenic activity. The mitogenic activity of B. adolescentis was also detected toward BALB/c nude (nu/nu) mouse splenocytes, as well as hetero (nu/+) and normal (+/+) mouse splenocytes. These results suggest that the high molecular weight immunopotentiator from B. adolescentis acted as a B cell mitogen, being different from lipopolysaccharide.
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Lee, J., Ametani, A., Sato, Y., Kaminogawa, S. (1993). Immunopotentiator from Bifidobacterium Adolescentis . In: Kaminogawa, S., Ametani, A., Hachimura, S. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2044-9_22
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DOI: https://doi.org/10.1007/978-94-011-2044-9_22
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