Abstract
High concentration cell cultures were conducted in a perfusion system based on external microfiltration with direct micron-sized bubble oxygenation. Kinetics of cell metabolism with respect to the consumption of glucose, glutamine and the production of lactate, ammonia and monoclonal antibody were examined and compared in different culture media. Cell-specific antibody production rate increased gradually as the cell concentration increased, whilst, cell-specific glucose, glutamine uptake rates and lactate, ammonia production rates decreased. Antibody production rate increased linearly with both the nutrient uptake rates and metabolite production rates in cultures without either nutrient limitation or metabolite inhibition. A stepwise decrease in cell concentration during the course of cultivation reversed the direction of the trends of changes in cell metabolic activities. Close relationship between the cell-specific metabolic rates and cell-specific growth rates was observed. The implications of the cellular metabolic information on the optimization of high concentration animal cell perfusion culture systems were discussed.
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Zhang, S., Handa-Corrigan, A., Spier, R.E. (1993). Kinetics of Cell Metabolism and Antibody Production in High Concentration Perfusion Cultures. In: Kaminogawa, S., Ametani, A., Hachimura, S. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2044-9_43
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DOI: https://doi.org/10.1007/978-94-011-2044-9_43
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