Abstract
Aspergillus niger has been grown in glucose-and maltose-limited recycling cultures to determine the maximum growth yield, the maximum product yield for glucoamylase production, and the maintenance requirements at very slow specific growth rates. Using the linear equation for substrate utilization, and using the experimental data from both recycling experiments,both the maximum growth yield,Yxsm,and the maximum product yield, Ypsm,could be determined. The values estimated were 157 g biomass per mol maltose for Yxsmand 100 g protein per mol maltose for Ypsm. Expressed on a C1-basis these values are 0.52 and 0.36 C-mole per C-mol for respectively Yxsmand Ypsm The found value for Ypsmis half the value found for alkaline serine protease production in Bacillus licheniformis, and it can be concluded that formation of extracellular protein is more energy consuming in filamentous fungi than in prokaryotic organisms. Maintenance requirements are no significant factor during growth of Aspergillus niger, and reported maintenance requirements are most probably due to differentiation.
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© 1992 Springer Science+Business Media Dordrecht
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van Verseveld, H.W., Metwally, M., el Sayed, M., Osman, M., Schrickx, J.M., Stouthamer, A.H. (1992). Determination of the maximum product yield from glucoamylase-producing Aspergillus niger grown in the recycling fermentor. In: Stouthamer, A.H. (eds) Quantitative Aspects of Growth and Metabolism of Microorganisms. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2446-1_13
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DOI: https://doi.org/10.1007/978-94-011-2446-1_13
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