Abstract
Successful expression of neurotransmitter receptors in heterologous systems in vitro requires that the host cell can synthesise adequate amounts of correctly processed and targeted protein. To date the most popular expression systems for receptors have been the injection of DNA or mRNA into Xenopus oocytes,1,2 or the use of vectors to transform Escherichia coli,3 yeast4 or mammalian cells, e. g. fibroblasts.5,6 One of the main disadvantages of these systems is that often only very small quantities of protein are synthesised, and in the case of E. coli, the proteins are often not correctly processed to allow functional analyses.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Marshall, J., Buckingham, S. D., Shingai, R., Lunt, G. G., Goosey, M. W., Darlison, M. G., Sattelle, D. B. & Barnard, E. A., Sequence and functional expression of a single α subunit of an insect nicotinic acetylcholine receptor. EMBO J., 9 (1990) 4391–4398.
Schofield, P. R., Darlison, M. G., Fujita, N., Burt, D. R., Stephenson, F. A., Rodriguez, H., Rhee, L. M., Ramachandran, J., Reale, V., Glencorse, T. A., Seeburg, P. H. & Barnard, E. A., Sequence and functional expression of the GABAA receptor shows a ligand-gated receptor super-family. Nature, 328 (1987) 221–227.
Gershoni, J. M., Expression of the α-bungarotoxin binding site of the nicotinic acetylcholine receptor by Escherichia coli transformants. Proc. Natl Acad. Sci. USA, 84 (1987) 4318–4321.
Fujita, N., Nelson, N., Fox, T. D., Claudio, T., Lindstrom, J., Riezman, H. & Hess, G. P., Biosynthesis of the Torpedo californica acetylcholine receptor α subunit in yeast. Science, 231 (1986) 1284–1287.
Blount, P., Smith, M. M. & Merlie, J. P., Assembly intermediates of the mouse muscle nicotinic acetylcholine receptor in stably transfected fibroblasts. J. Cell. Biol., 111 (1990) 2601–2611.
Claudio, T., Green, W. N., Hartman, D. S., Hayden, D., Paulson, H. L., Sigworth, F. J., Sine, S. M. & Swedlund, A., Genetic reconstitution of functional acetylcholine receptor channels in mouse fibroblasts. Science, 238 (1987) 1688–1694.
Miller, L. K., Insect baculoviruses: powerful gene expression vectors. BioEssays, 4 (1989) 91–95.
Cameron, I. R., Possee, R. D. & Bishop, D. H. L., Insect cell culture technology in baculovirus expression systems. TIBTech., 7 (1989) 66–70.
Atkinson, A. E., Weitzman, M. D., Obosi, L. A., Beadle, D. J. & King, L. A., Baculo viruses as vectors for foreign gene expression in insect cells. Pestic. Sci., 28 (1990) 215–224.
Bishop, D. H. L. & Possee, R. D., Baculovirus expression vectors. Adv. Gene Technol., 1 (1990) 55–72.
Weyer, U., Knight, S. & Possee, R. D., Analysis of very late gene expression by Autographa calif ornica nuclear polyhedrosis virus and the further development of multiple expression vectors. J. Gen. Virol., 71 (1990) 1525–1534.
Klaiber, K., Williams, N., Roberts, T. M., Papazian, D. M., Jan, L. Y. & Miller, C., Functional expression of Shaker K+ channels in baculovirus-infected insect cell line. Neuron, 5 (1990) 221–226.
Hill-Perkins, M. S, & Possee, R. D., A baculovirus expression vector derived from the basic protein promoter of Autographa californica nuclear polyhedrosis virus. J. Gen. Virol., 71 (1990) 971–976.
Martignoni, M. E. & Iwai, P. J., A catalogue of viral diseases of insects, mites and ticks. USDA Forest Service PNW-195, Washington, DC: USGPO.
Vaughan, J. L., Goodwin, R. H., Tompkins, G. J. & McCawley, P., The establishment of two insect cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro, 13 (1977) 213–217.
Maeda, S., Expression of foreign genes in insects using baculovirus vectors. Ann. Entomol., 34 (1989) 351–372.
Rohrmann, G. F., Polyhedrin structure. J. Gen. Virol., 67 (1986) 1499–1513.
Smith, G. E., Fraser, M. J. & Summers, M. D., Molecular engineering of the Autographa californica nuclear polyhedrosis virus genome: deletion mutants within the polyhedrin gene. J. Virol., 46 (1983) 584–593.
Kitts, P. A., Ayres, M. D. & Possee, R. D., Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors. Nucleic Acid. Res., 18 (1990) 5667–5672.
Possee, R. D. & Howard, S. C, Analysis of the polyhedrin gene promoter of the Autographa californica nuclear polyhedrosis virus. Nucleic Acid Res., 15 (1987) 10233–10248.
Miller, D. W., Safer, P. & Miller, L. K., An insect baculovirus host-vector system for high-level expression of foreign genes. In Genetic Engineering, Vol. 8, ed. J. K. Setlow & A. Hollaender. Plenum, New York, 1986, pp. 277–298.
Matsuura, Y., Possee, R. D. & Bishop, D. H. L., Expression of the S-coded genes of lymphocytic choriomeningitis arenavirus using a baculovirus vector. J. Gen. Virol., 67 (1986) 1515–1529.
Carbonell, L. F., Hodge, M. R., Tomalski, M. D. & Miller, L. K., Synthesis of a gene coding for an insect-specific scorpian neurotoxin and attempts to express it using baculovirus vectors. Gene, 73 (1988) 409–418.
Weyer, U. & Possee, R. D., Analysis of the promoter of the Autographa californica nuclear polyhedrosis virus p10 gene. J. Gen. Virol., 70 (1989) 203–208.
Luckow, V. A., Cloning and expression of heterologous genes in insect cells with baculovirus vectors. In Recombinant DNA Technology and Applications, ed. C. Ho, A. Prokop & R. Bajpai. McGraw-Hill, New York, 1990, pp. 97–152.
Butters, T. D. & Hughes, R. C, Isolation and characterization of mosquito cell membrane glycoproteins. Biochim. Biophys. Acta, 640 (1981) 655–671.
Butters, T. D., Hughes, R. C. & Visher, R. C, Steps in the biosynthesis of mosquito cell membrane glycoproteins and the effects of tunicamycin. Biochim. Biophys. Acta, 640 (1981) 672–686.
Jarvis, D. L., Oker-Blom, C. & Summers, M. D., Role of glycosylation in the transport of recombinant glycoproteins through the secretory pathways of lepidop-terian insect cells. J. Cell. Biochem., 42 (1990) 181–191.
Armstrong, C. M. & Miller, C, Do voltage-dependent K+ channels require Ca2+? A critical test employing a heterologous expression system. Proc. Natl Acad. Sci. USA, 87 (1990) 7579–7582.
Birnbaumer, L., Abramowitz, J. & Brown, A. M., Receptor-effector coupling by G proteins. Biochim. Biophys. Acta, 1031 (1990) 163–224.
Luckow, V. A. & Summers, M. D., Trends in the development of baculovirus expression vectors. Bio/Technol., 6 (1988) 47–55.
Reilander, H., Boege, F., Vasudevan, S., Maul, G., Hekman, M., Dees, C., Hampe, W., Halmreich, E. J. M. & Hartmut, M., Purification and functional expression of the human β2-adrenergic receptor produced in baculovirus-infected insect cells. FEBS, 282 (1991) 441–444.
Atkinson, A. E., Earley, F. G. P., Beadle, D. J. & King, L. A., Expression and characterization of the chick nicotinic acetylcholine receptor α-subunit in insect cells using a baculovirus vector. Eur. J. Biochem., 192 (1990) 451–458.
King, L. A. & Possee, R. D., The Baculovirus Expression Vector System: a Laboratory Guide. Chapman and Hall, London, 1992.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 SCI
About this chapter
Cite this chapter
King, L.A., Atkinson, A.E., Obosi, L.A., Bermudez, I., Beadle, D.J. (1992). Expression of Neurotransmitter Receptors in Insect Cells Using Baculovirus Vectors. In: Duce, I.R. (eds) Neurotox ’91. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2898-8_14
Download citation
DOI: https://doi.org/10.1007/978-94-011-2898-8_14
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-85166-746-8
Online ISBN: 978-94-011-2898-8
eBook Packages: Springer Book Archive