Skip to main content

The Use of Bulked Segregant Analysis to Identify AFLP Molecular Markers Closely Linked to Melampsora Larici-Populina Resistance in Populus

  • Chapter
Somatic Cell Genetics and Molecular Genetics of Trees

Part of the book series: Forestry Sciences ((FOSC,volume 49))

  • 136 Accesses

Abstract

We have identified three AFLP markers tightly linked to the locus conferring resistance to the leaf rust Melampsora larici-populina in Populus by Bulked Segregant Analysis. The study was carried out using a hybrid progeny derived from an inter-specific, controlled cross between a resistant Populus deltoides female and a susceptible P. nigra male. The segregation ratio of resistant to susceptible plants suggested that a single, dominant locus (Mer) defined the resistance to Melampsora.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bradshaw, H.D. Jr, Villar, M., Watson, B.D., Otto, K.G., Stewart, S., and Settler, R.F. (1994). Molecular genetics of growth and development in Populus. III. A genetic linkage map of a hybrid poplar composed of RFLP, STS, and RAPD markers. Theor. Appl. Genet. 89, 167–178.

    CAS  Google Scholar 

  • Bradshaw, H.D. Jr. and Stettler, R.F. (1995a) Molecular genetics of growth and development in Populus. IV. Mapping QTLs with large effects on growth, and phenology traits in a forest tree. Genetics 139, 963–973.

    PubMed  CAS  Google Scholar 

  • Bradshaw, H.D. Jr. and Stettler, R.F. (1995b) Field trials to identify quantitative trait loci underlying growth, morphology, and stress response in Populus. Abstract presented at the International Poplar Symposium, Seattle (WA, USA), August 20-25, 1995.

    Google Scholar 

  • Dellaporta, S.L., Wood, J. and Hicks, J.B. (1983) A plant DNA minip reparation: Version II. Plant Mol. Biol. Rep. 1, 19–21.

    Article  CAS  Google Scholar 

  • Kosambi, D.D. (1944) The estimation of map distances from recombination values. Ann. Eugen. 12, 172–175.

    Google Scholar 

  • Mather, K. (1938) The Measurement of Linkage in Heredity (Methuen’s Monographs on Biological Subjects). Methuen, London.

    Google Scholar 

  • Michelmore, R.W., Paran, I. and Kesseli, R.V. (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. USA 88, 9828–9832.

    Article  PubMed  CAS  Google Scholar 

  • Neale, D.B. and Harry, D.E. (1994) Genetic mapping in forest trees: RFLPs, RAPDs and beyond. AgBiotech. News Inform. 6, 107N–114N.

    Google Scholar 

  • Sax, K. (1923) The association of size differences with seed-coat pattern and pigmentation in Phaseolus vulgaris.Genetics 8, 552–560.

    PubMed  CAS  Google Scholar 

  • Tanksley, S.D., Ganal, M.W. and Martin, G.B. (1995) Chromosomelanding: a paradigm for map-based gene cloning in plants with large genomes. Trends Genet. 11, 63–68.

    Article  PubMed  CAS  Google Scholar 

  • Thomas, CM., Vos, P., Zabeau, M., Jones, D.A., Norcott, K.A., Chadwick, B.P. and Jones, D.J.G. (1995) Identification of amplified restriction fragment polymorphism (AFLP) marker tightly linked to the tomato Cf-9 gene for resistance to Cladosporium fulvum.Plant J. 8, 785–794.

    Article  PubMed  CAS  Google Scholar 

  • Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Homes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M. and Zabeau, M. (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23, 4407–4414.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1996 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Cervera, MT. et al. (1996). The Use of Bulked Segregant Analysis to Identify AFLP Molecular Markers Closely Linked to Melampsora Larici-Populina Resistance in Populus . In: Ahuja, M.R., Boerjan, W., Neale, D.B. (eds) Somatic Cell Genetics and Molecular Genetics of Trees. Forestry Sciences, vol 49. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3983-0_28

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-3983-0_28

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5761-5

  • Online ISBN: 978-94-011-3983-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics