Skip to main content

Biological and Chemical Evaluation of Plant Extracts and Subsequent Isolation Strategy

  • Chapter
Bioassay Methods in Natural Product Research and Drug Development

Part of the book series: Proceedings of the Phytochemical Society of Europe ((PPSE,volume 43))

Abstract

More than 85% of higher plants have not been adequately surveyed for potentially useful biological activity and the plant kingdom has not received sufficient attention as a resource of possible medicinal agents. At the same time, it has been estimated that over half of the world’s 25 best selling pharmaceuticals for 1991 owed their origin to a natural source material (O’Neill and Lewis, 1993). Another statistic is that approximately 120 plant-derived chemical compounds are currently used as drugs. Many of these are extracted and purified directly from plants (Farnsworth et al, 1985). A recent success story is the market for Ginkgo biloba, (Ginkgoaceae) extracts in disorders of peripheral blood circulation and geriatry. Annual sales in Europe are estimated at US $ 500 million (Sticher 1993).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 189.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Albert, K. (1995) On-line use of NMR detection in separation chemistry, J. Chromatogr. A, 703, 123–147.

    Article  CAS  Google Scholar 

  • Cuendet M., Hostettmann K., Potterat O., and Dyatmiko, W. (1997) Iridoid glucosides with free radical scavenging properties from Fagraea blumei, Helv. Chim. Acta, 80

    Google Scholar 

  • Farnsworth, N.R., Akerele, O, Bingel, A.S., Soejarto, D.D., and Guo, Z.G. (1985) Medicinal plants in therapy, Bull.W.H.O. 63, 965–9

    CAS  Google Scholar 

  • Hamburger, M.O., and Cordell, G.A. (1987) A direct bioautographic TLC assay for compounds possessing antibacterial activity, J. Nat. Prod., 50, 19–22.

    Article  CAS  Google Scholar 

  • Homans, A.L., and Fuchs, A. (1970) Direct bioautography on thin-layer chromatograms as a method for detecting fungitoxic substances, J. Chromatogr., 51, 325–327.

    Article  Google Scholar 

  • Hostettmann K., and Marston, A. (1990) Bioactive constituents of plants used in African traditional medicine, in Atta-ur-Rahman (ed.), Studies in Natural Products Chemistry, Vol., 7, Elsevier, Amsterdam, pp. 405–437.

    Google Scholar 

  • Hostettmann K., Domon B., Schaufelberger D., and Hostettmann, M. (1984) On-line high-performance liquid chromatography: ultraviolet-visible spectroscopy of phenolic compounds in plant extracts using postcolumn derivatization, J. Chromatogr., 283, 137–147.

    Article  CAS  Google Scholar 

  • Hostettmann K., Marston A., and Wolfender J.-L. (1995) Strategy in the search for new biologically active plant constituents, in K. Hostettmann, A. Marston, Maillard and M. Hamburger (eds.), Phytochemistry of Plants Used in Traditional Medicine, Clarendon Press, Oxford, pp. 17–45.

    Google Scholar 

  • Hostettmann K., Wolfender, J.-L., and Rodriguez, S. (1997a) Rapid detection and subsequent isolation of bioactive constituents of crude plant extracts, Planta Med., 63, 2–10.

    Article  CAS  Google Scholar 

  • Hostettmann K., Terreaux, C, Marston A., and Potterat, O. (1997b) The role of planar chromatography in the rapid screening and isolation of bioactive compounds from medicinal plants, J. Planar Chromatogr., 10, 251–257.

    CAS  Google Scholar 

  • Marston A., and Hostettmann, K. (1994) Counter-current chromatography as a preparative tool-applications and perspectives, J. Chromatogr. A, 658, 315–341.

    Article  CAS  Google Scholar 

  • Marston A., Maillard M., and Hostettmann, K. (1997) The role of TLC in the investigations of medicinal plants of Africa, South America and other tropical regions, GIT Laboratory Journal No., 1, 36–39.

    Google Scholar 

  • O’Neill, M.J. and Lewis, J.A. (1993) The renaissance of plant research in the pharmaceutical industry, in A.D. Kinghorn and M.F. Balandrin (eds.), Human Medicinal Agents from Plants, American Chemical Society, Washington, DC, pp. 48–55.

    Chapter  Google Scholar 

  • Rahalison L., Hamburger M., Hostettmann K., Monod M., and Frenk, E. (1991) A bioautographic agar overlay method for the detection of antifungal compounds from higher plants, Phytochem. Anal., 2, 199–203.

    Article  CAS  Google Scholar 

  • Rath G., Potterat O., Mavi S., and Hostettmann, K. (1996) Xanthones from Hypericum roeperanum, Phytochemistry, 43, 513–520.

    Article  CAS  Google Scholar 

  • Rios, J.L., Recio, M.C., and Villar, A. (1988) Screening methods for natural products with antimicrobial activity: a review of the literature, J. Ethnopharmacol., 23, 127–149.

    Google Scholar 

  • Rodriguez S., Wolfender, J.-L., Hakizamungu E., and Hostettmann, K. (1995) An antifungal naphthoquinone, xanthones and secoiridoids from Swertia calycina, Planta Med., 61, 362–364.

    Article  CAS  Google Scholar 

  • Saxena G., Farmer S., Towers, G.H.N., and Hancock, R.E.W. (1995) Use of specific dyes in the detection of antimicrobial compounds from crude plant extracts using a thin layer chromatography agar overlay technique, Phytochem. Anal., 6, 125–12

    Article  CAS  Google Scholar 

  • Spraul M., Hoffmann M., Lindon, J.C., Nicholson, J.K., and Wilson, I.D. (1993) Liquid chromatography coupled with high field proton nuclear magnetic resonance spectroscopy: Current status and future prospects, Analytical Proceedings, 30

    Google Scholar 

  • Sticher, O. (1993) Quality of Ginkgo, preparations, Planta Med., 59, 2–11.

    Article  CAS  Google Scholar 

  • Terreaux C., Maillard M., Hostettmann K., Lodi G., and Hakizamungu, E. (1994) Analysis of the fungicidal constituents from the bark of Ocotea usambarensis, Engl. (Lauraceae), Phytochem. Anal., 5, 233–238.

    Article  Google Scholar 

  • Watt, J.M., and Breyer-Brandwijk, M.G. (1962) The Medicinal and Poisonous Plants of outhern and Eastern Africa, E. and S. Livingstone, Edinburgh.

    Google Scholar 

  • Wolfender, J.-L., and Hostettmann, K. (1995) Applications of liquid chromatography-mass spectrometry in the investigation of medicinal plants, in J.T. Arnason, R. Mata, Romeo (eds.), Phytochemistry of Medicinal Plants, Plenum Press, New York, 189–215.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1999 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Marston, A., Hostettmann, K. (1999). Biological and Chemical Evaluation of Plant Extracts and Subsequent Isolation Strategy. In: Bohlin, L., Bruhn, J.G. (eds) Bioassay Methods in Natural Product Research and Drug Development. Proceedings of the Phytochemical Society of Europe, vol 43. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4810-8_6

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-4810-8_6

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6019-6

  • Online ISBN: 978-94-011-4810-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics