Abstract
A polyclonal antibody toward the borate-rhamnogalacturonan-II (RG-II) complex was raised in rabbits. The antibody recognizes RG-II exclusively in cell walls. Immunocytochemical studies reveal that the epitope is ubiquitous in cell walls of all the cells in radish (Rhaphanus sativus) roots. The label was denser proximal to the plasma membrane and was not detected in the middle lamella. These results suggests that the borate bridge between RG-II chains is important for the network formation of newly produced pectic polysaccharides, and that the bridge may not be involved in cell to cell adhesion.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Darvill A G, McNeil M and Albersheim P 1978 Structure of plant cell walls VIII. A new pectic polysaccharide. Plant Physiol. 62, 418–422.
Freshour G, Clay R P, Fuller M S, Albersheim P, Darvill A G and Hahn M G 1996 Developmental and tissue-specific structural alterations of the cell-wall polysaccharides of Arabidopsis thaliana roots. Plant Physiol. 110, 1413–1429.
Ishii T and Matsunaga T 1996 Isolation and characterization of a boron rhamnogalacturonan-II complex from cell walls of sugar beet pulp. Carbohydr. Res. 284, 1–9.
Jarvis M C 1982 The proportion of calcium-bound pectin in plant cell walls. Planta 154. 344–346.
Knox J P, Linstead P J, King J, Cooper C and Roberts K 1990 Pectin esterification is spatially regulated both with in cell walls and between developing tissues of root apices. Planta 181, 512–521.
Kobayashi M, Matoh T and Azuma J 1995 Structure and glycosyl composition of the boron-polysaccharide complex of radish roots. Plant Cell Physiol. 36S, 139.
Kobayashi M, Matoh T and Azuma J 1996 Two chains of rhamnogalacturonan II are cross-linked by borate-diol ester bonds in higher plant cell walls. Plant Physiol. 110, 1017–1020.
Kobayashi M, Ohno K and Matoh T 1997 Boron nutrition of cultured tobacco BY-2 cells. II. Characterization of the boron-polysaccharide complex. Plant Cell Physiol. 38, 676–683.
Liners F and Van Cutsem P 1992 Distribution of pectic polysaccharides throughout walls of suspension-cultured carrot cells. An immunocytochemical study. Protoplasma 170, 10–21.
Liners F, Letesson J-J, Didembourg C and Van Cutsem P 1989 Monoclonal antibodies against pectin, Recognition of a conformation induced by calcium. Plant Physiol. 91, 1419–1424.
Loomis W D and Durst R W 1992 Chemistry and biology of boron. BioFactors 3, 229–239.
Matoh T, Kawaguchi S and Kobayashi M 1996 Ubiquity of a borate rhamnogalacturonan II complex in the cell walls of higher plants. Plant Cell Physiol. 37, 636–640.
Matoh T, Ishigaki K, Ohno K and Azuma J 1993 Isolation and characterization of a boron-polysaccharide complex from radish roots. Plant Cell Physiol. 34, 639–642.
Moore P J and Staehelin L A 1988 lmmunogold localization of the cell-wall matrix polysaccharides rhamnogalacturonan I and xyloglucan during cell expansion and cytokinesis in Trifolium pratense L.; implication for secretory pathways. Planta 174, 433–445.
Moore P J, Darvill A G, Albersheim P and Staehelin L A 1986 Immunogold localization of xyloglucan and rhamnogalacturonan I in the cell walls of suspension-cultured sycamore cells. Plant Physiol. 82, 787–794.
O’Neill M A, Warrenfeltz D, Kates K, Pellerin P, Doco T, Darvill A G and Albersheim P 1996 Rhamnogalacturonan-II, a pectic polysaccharide in the walls of growing plant cells, forms a dimer that is covalently cross-linked by a borate ester. J. Biol. Chem. 271, 22923–22930.
Pellerin P, Doco T, Vidal S, Williams P, Brillouet J-M and O’Neill M A 1996 Structural characterization of red wine rhamnogalacturonan II. Carbohydr. Res. 290, 183–197.
Spurr A R 1957 The effect of boron on cell-wall structure in celery. Am. J. Bot. 44, 637–650.
Stevenson T T, Darvill A G and Albersheim P 1988 Structural features of the plant cell wall polysaccharide rhamnogalacturonan-II. Carbohydr. Res. 182, 207–226.
Williams M N V, Freshour G, Darvill A G, Albersheim P, and Hahn M G 1996 An antibody Fab selected from a recombinant phage display library detects deesterified pectic polysaccharide rhamnogalacturonan H in plant cells. Plant Cell 8, 673–685.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Takasaki, M., Kawaguchi, S., Kobayashi, M., Takabe, K., Matoh, T. (1997). Immunocytochemistry of the borate-rhamnogalacturonan II complex in cell walls of radish roots. In: Bell, R.W., Rerkasem, B. (eds) Boron in Soils and Plants. Developments in Plant and Soil Sciences, vol 76. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5564-9_46
Download citation
DOI: https://doi.org/10.1007/978-94-011-5564-9_46
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6344-9
Online ISBN: 978-94-011-5564-9
eBook Packages: Springer Book Archive