Abstract
Several types of bioreactors were incorporated to verify the effectiveness for mass culture of ornamental plants. Protocorm-like bodies (PLBs) were proliferated from flower stalk of Phalaenopsis by using bioreactors. Hyponex medium containing 1% potato and 0.1% activated charcoal supplied by ebb-and-flow method was investigated as the best for PLBs production (17 or more per PLB). The result partially suggested the possibility for mass propagation of PLBs through bioreactor system. Several cultivars of Lilium were successfully mass propagated using different types of bioreactors. In the case of ‘Marcopolo’, the growth of bulblets in balloon type bubble bioreactor (BTBB) was nearly 10 folds faster than that of the solid medium. When 120 single-nodal expiants (about 1 cm in length) were cultured for 8 weeks under 16hr light condition, more than 20,000 shoot cuttings of virus-free potatoes could be obtained in 20 L sized BTBB. The percentage of ex vitro rooting of the cuttings during weaning stage revealed as more than 95% under 50% shade. Similar results were obtained from the culture of single-nodal expiants using bioreactor in chrysanthemum and sweet potato. Based on our above results, we suggest that large-scale cultures of plant cell, tissue and organ using bioreactor system should be quite a feasible approach in terms of increase of multiplication rate and reduction of production cost when compared with conventional plant tissue culture method.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Aitken-Christie, J. 1991. Automation, In: P.C. Debergh and R.H. Zimmerman (eds.). Micropropagation. Kluwer Academic Publishers, Dordrecht, pp. 342–354.
Jones, J.B. and C.J. Sluis. 1991. Marketing of micropropagated plants. In: PC. Debergh and R.H. Zimmerman (eds.). Micropropagation. Kluwer Academic Publishers, Dordrecht, pp. 141–154.
Kim, Y.S. 1999. Development of large scale process for mass production of lily bulblet by bioreactor system. MS thesis in Chungbuk National Univ. Korea.
Leathers, R.R., M.A.L. Smith and J. Aiken-Christie. 1995. Automation of the bioreactor process for mass propagation and secondary metabolism In: J. Aitken-Christie, T. Kozai and M.A.L. Smith (eds.). Automation and Environmental Control in Plant Tissue Culture. Kluwer Academic Publishers, Netherlands, pp. 187–214.
Lee, J.S. 1999. Micropropagation of Phalaenopsis by flower stalk-derived axillary bud culture. MS Thesis, Chungbuk National Univ. Korea.
Levin, R., V. Gaba, B. Tal, S. Hirsch, D. De Nola and I.K. Vasil. 1988. Automated tissue culture for mass propagation. Bio/Technology 6:1035–1040.
Lim, S., J.H. Seon, K.Y. Paek, S.H. Son and B.H. Han. 1998. Development of pilot scale process for mass production of Lilium bulblets in vitro. Acta. Hort. 461:237–241.
Nishimura, S., T. Terashima, K. Higashi and H. Kamada. 1993. Bioreactor culture of somatic embryos for mass propagation of plants In; Redenbaugh, K (ed.). Synseeds-Applications of Synthetic Seeds to Crop Improvement. CRC Press. London/Tokyo, pp. 175–181.
Preil, W. 1991. Application of bioreactors in plant propagation. In: P.C. Debergh and R.H. Zimmerman (eds.). Micropropagation. Kluwer Academic Publishers, Netherland. pp. 425–445.
Preil, W., P. Florek, U. Wix and A. Beck. 1988. Towards mass propagation by use of bioreactors. Acta Hort. 226:99–105.
Rittershaus, E., J. Ulrich, A. Weiss and K. Westphal. 1989. Large scale industrial fermentation of plant cells: Experiences in cultivation of plant cells in a fermentation cascade up to a volume of 75,000 liters. Bio Engineering. 5:28–34.
Seon, J.H., K.W. Yoo, Y.Y. Cui, M.H. Kim, S.J. Lee, S.H. Son and K.Y. Paek. 1998. Application of bioreactor for the production of saponin by adventitious root cultures in Panax ginseng. In: A. Altman, M. Ziv and S. Izhar (eds.). Plant Biotechnology and In Vitro Biology in the 21st Century. Kluwer Academic Plublishers. Netherlands, pp. 329–332.
Smart, N.J. and M.W. Fowler. 1984. An airlift column bioreactor suitable for large-scale cultivation of plant cell suspensions. EXP. Bot. 35:531–537.
Son, S.H., S.M. Choi, D.S. Lee, S.R. Yun and K.Y. Paek. 1999a. Commercial application of mountain ginseng through bioreactor culture system. Proc. Korea-Japan Joint Sym. Transplant Production in Horticultural Plants. Res. Center for the Development of Advanced Horticultural Technology, Changbuk National Univ.
Son, S.H., S.M. Choi, S.R. Kwon, Y.H. Lee and K.Y Paek. 1999b. Large-scale culture of plant cell and tissue by bioreactor system. J. Plant Bioteh. 1(1): 1–8.
Takahashi, S., H. Matsubara, H. Yamagata and T. Morimoto. 1992. Micropropagation of virus free bulblets of Lilium longiflorum by tank culture. 1. Development of liquid culture method and large scale propagation. Acta Hort. 319:83–88.
Thomas, D. and T. Murashige. 1979. Volatile emissions of plant tissue cultures. I Identification of the major components. In Vitro 15:654–658.
Tulecke, W. and L.G. Nickell. 1959. Production of large amounts of plant tissue by submerged culture. Science. 130:863–864.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Paek, K.Y., Hahn, EJ., Heo, J., Son, S.H. (2000). Micropropagation of Ornamental Plants Using Bioreactor System. In: Kubota, C., Chun, C. (eds) Transplant Production in the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9371-7_41
Download citation
DOI: https://doi.org/10.1007/978-94-015-9371-7_41
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5570-5
Online ISBN: 978-94-015-9371-7
eBook Packages: Springer Book Archive