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General Strategies

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Metabolic Engineering of Plant Secondary Metabolism

Abstract

In the past years different methods have been developed for the genetic transformation of plants and plant cells. The two most successful ones are direct introduction of genes using the particle gun and via a (binary) vector transferred by Agrobacterium. In the Chapters 3 and 4 these methods and some applications are described in more detail. Agrobacterium tumefaciens is widely used for transformation of plants. A major restriction is the limited host range, which is mostly dicotyledonous plants, and even in this group not all plants. The particle gun is, in principle, applicable to any plant species. Though transformation is feasible, the regeneration of a plant from the transformed cells is often a difficult task.

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  1. R. Verpoorte
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  2. R. Van Der Heijden
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  3. J. Memelink
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Editors and Affiliations

  1. Division of Pharmacognosy, Leiden/Amstredam Center for Drug Research, Einsteinweg 55, P.O. Box 9502, 2300 RA, Leiden, The Netherlands

    Robert Verpoorte

  2. Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Düsseldorf, Universitätsstr. 1, Geb. 26.13, 40225, Düsseldorf, Germany

    A. W. Alfermann

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Verpoorte, R., Van Der Heijden, R., Memelink, J. (2000). General Strategies. In: Verpoorte, R., Alfermann, A.W. (eds) Metabolic Engineering of Plant Secondary Metabolism. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9423-3_2

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  • DOI: https://doi.org/10.1007/978-94-015-9423-3_2

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