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In Vitro Regeneration and Genetic Transformation of Lentil

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Applied Genetics of Leguminosae Biotechnology

Part of the book series: Focus on Biotechnology ((FOBI,volume 10B))

Abstract

Lentil (Lens culinaris Medik.) is an important pulse crop in many diverse regions around the world. As such, farmers face diverse agronomic problems that have not been adequately addressed using conventional plant breeding methods. Biotechnology holds the promise of overcoming many problems with the lentil crop, including insect resistance and disease tolerance, weed control, nutritional enhancements and reduction of antinutri-tional components. In order to apply biotechnology to lentil crop improvement, however, the underlying technologies, including tissue culture, in vitro regeneration, and gene transfer technologies, need to be elucidated and formalized. In this chapter we review the history and the current state of biotechnology relating to lentil. Like many grain legumes, lentil has a long but often frustrating history of tissue culture and regeneration in vitro. Lentil plant regeneration has been reported using both direct and indirect organogenic processes, as well as via somatic embryogenesis. We analyze the important factors in the tissue culture and regeneration of lentil in vitro. Gene transfer into lentil has been attempted using Agrobacterium based techniques and also by particle gun techniques. We also discuss the factors influencing gene transfer technology for lentil and prospects for future successes.

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Authors and Affiliations

  1. Crop Development Centre, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, S7N 5A8, Canada

    Anju Gulati & Alan McHughen

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  1. Anju Gulati
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  2. Alan McHughen
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Editors and Affiliations

  1. Department of Biosciences, M.D. University, Rohtak, 124001, India

    Pawan K. Jaiwal  & Rana P. Singh  & 

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Gulati, A., McHughen, A. (2003). In Vitro Regeneration and Genetic Transformation of Lentil. In: Jaiwal, P.K., Singh, R.P. (eds) Applied Genetics of Leguminosae Biotechnology. Focus on Biotechnology, vol 10B. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0139-6_8

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  • DOI: https://doi.org/10.1007/978-94-017-0139-6_8

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-6369-4

  • Online ISBN: 978-94-017-0139-6

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