Abstract
The successful implementation of gene therapy approaches in the clinic still awaits the development of optimized processes for the manufacturing of gene transfer vectors. To date, a majority of clinical trials have been using vectors derived from Murine Leukemia Viruses (MLV) (Andreadis et al. 1999, http://www.wiley.eo.uk /genmed). Despite of their limitations (i.e. relatively low titer and activity, use limited to dividing cells), MLV vectors lead to permanent gene transfer and expression and the first clinical success of gene therapy has been obtained using this technology (Cavazzana-Calvo et al. 2000).
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Gény-Fiamma, C., Millot, L., Rocca, C., Danos, O., Merten, OW. (2003). Optimization of the Production of Retroviral Vectors. In: Yagasaki, K., Miura, Y., Hatori, M., Nomura, Y. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0726-8_16
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