Abstract
Total chromosomal DNAs from 20 Bradyrhizobium spp. strains (10 strains isolated from Vigna radiata and 10 from Arachis hypogaea) and 18 B. japonicum strains isolated from Glycine max were extracted. These DNAs served as templates for REP, ERIC and RAPD primers in PCR analyses. The patterns of the resulting PCR products were analyzed and highly specific for each strain, especially when grouped together with their antibiotic-resistance profiles. A method for extracting DNA directly from soil was developed. Recovery was approximately 30 µg DNA g−1 soil and the procedure yielded DNA suitable for PCR amplification.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Beringer J E 1974 R-factor transfer in Rhizobium leguminosarum. J. Gen Microbiol. 84, 188–198.
Bruijn De F J 1993 Use of Repetitive (Repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus) sequences and polymerase chain reaction to fingerprint the genomes of Rhizobium meliloti isolates and other soil bacteria. Appl. Environ. Microbiol. 58, 2180–2187.
Elkan G H 1992 Taxonomy of the Rhizobia. Can. J. Microbiol. 38, 446–450.
Hilger A B and Myrold D D 1991 Method for extraction of Frankia DNA from soil. Agric. Ecosystems. Environ. 34, 107–113.
Holben W E, Schroeter B M, Calabrese V G M, Olsen R H, Kukor J K, Biederbeck V O, Smith A E and Tiedje J M 1992 Gene probe analysis of soil microbial populations selected by amendment with 2,4-dichlorophenoxyacetic acid. Appl. Environ. Microbiol. 58, 3941–3948.
Holben W E, Jansson J K, Chelm B K and Tiedje J M 1988 DNA probe method for detection of specific microorganism in the soil bacterial community. Appl. Environ. Microbiol. 54, 703–711.
Ingraham J L, Maaloc O and Neidhardt F C 1983 Growth of the Bacterial Cell. Sinaucr Associates, Inc., Sunderland, MA. pp 148.
Jacobsen S C and Rasmussen O F 1992 Development and application of a new method to extract bacterial DNA from soil based on separation of bacterial from soil with cation-exchange resin. Appl. Environ. Microbiol. 58, 2458–2462.
Judd K A, Schneider G M, Sadowsky M J and de Bruijn F J 1993 Use of repetitive sequences and the polymerase chain reaction technique to classify genetically related Bradyrhizobium japonicum serocluster 123 strains. Appl. Environ. Microbiol. 59, 1702–1708.
Keyser H H and Cregan P B 1987 Nodulation and competition for nodulation of selected soybean genotypes among Bradyrhizobium japonicum scrogroup 123 isolates. Appl. Environ. Microbiol. 53, 2631–2635.
Kuykendall L D, Roy M A, O’Neill J J and Devine T E 1988 Fatty acids, antibiotic resistance and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int. J. Syst. Bacterial. 38, 358–361.
Langridge L, Langridge P and Bergquist L 1980 Extraction of nucleic acids from agarose gels. Anal. Biochem. 103, 264–271.
McCoy W F and Olson B 1995 Fluorometric determination of DNA concentration in municipal drinking water. Appl. Environ. Microbiol. 49, 811–817.
Nick G and Lindstrom K 1994 Use of repetitive sequences and the polymerase chain reaction to fingerprint the genomic DNA of Rhizobium galegae strains and to identify the DNA obtained by sonicating the liquid cultures and root nodules. System. Appl. Microbiol. 17, 265–273.
Picard C, Ponsonnet C, Paget E, Nesme X and Simonet P 1992 Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction. Appl. Environ. Microbiol. 58, 2717–2722.
Sayler S G and Layton A C 1990 Environmental application of nucleic acid hybridization. Annu. Rev. Microbiol. 44, 625–648.
Trevors J T and van Elsas J D 1989 A review of selected methods in environmental microbial genetics. Can. J. Microbiol. 35, 895901.
Versalovic J, Koeuth T and Lupski J R 1991 Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucl. Acids Res. 19, 6823–6831.
Williams G K J, Kubelik A N, Livak K J, Rafalski J A and Tingey S V 1993 DNA polymorphisms amplified by arbitrary primers arc useful as genetic marker. Nucl. Acids Res. 18, 6531–66535.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Teaumroong, N., Boonkerd, N. (1998). Detection of Bradyrhizobium spp. and B. japonicum in Thailand by primer-based technology and direct DNA extraction. In: Hardarson, G., Broughton, W.J. (eds) Molecular Microbial Ecology of the Soil. Developments in Plant and Soil Sciences, vol 83. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2321-3_12
Download citation
DOI: https://doi.org/10.1007/978-94-017-2321-3_12
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5099-1
Online ISBN: 978-94-017-2321-3
eBook Packages: Springer Book Archive