Abstract
Leek plants (A Ilium porrum L.), infected or not with the arbuscular mycorrhizal (AM) fungus, Glomus mosseae, were grown in a sand-hydroponic system, fed with a nutrient solution containing 3.2 or 96 µM P and analyzed for root IAA and ZR content, to assess the role played by the fungus and P nutrition on host hormonal balance. IAA was analyzed by HPLC-fluorimetry, ZR by HPLC-UV coupled with a bioassay based on the expression of a phytohormone-regulated GUS reporter gene. Shoot and root weights and shoot FW-DW ratio enhancements, as well as root-to-shoot DW ratio decrement in mycorrhizal plants, were related to P nutrition. Shoot P concentration was increased by mycorrhizae at both P levels, but was comparable in AM plants grown at 3.2 µM P and non mycorrhizal (NM) plants at 96 µM P. Mycorrhizae and P increased IAA at substantially similar values, while P increased ZR much more than mycorrhizae did. These results are discussed in relation to root architecture modifications induced by the AM fungus.
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Abbreviations
- AM:
-
arbuscular mycorrhizal
- DW:
-
dry weight
- FW:
-
fresh weight
- IAA:
-
indole-acetic acid
- IPA:
-
indole-propionic acid
- MU:
-
4-methyl-umbelliferyl-glucuronide
- NAA:
-
naphthalene-acetic acid
- NM:
-
non mycorrhizal
- P:
-
phosphorus
- ZR:
-
zeatin ribo side
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Torelli, A., Trotta, A., Acerbi, L., Arcidiacono, G., Berta, G., Branca, C. (2001). IAA and ZR content in leek (Allium porrum L.), as influenced by P nutrition and arbuscular mycorrhizae, in relation to plant development. In: Gašparíková, O., Čiamporová, M., Mistrík, I., Baluška, F. (eds) Recent Advances of Plant Root Structure and Function. Developments in Plant and Soil Sciences, vol 90. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2858-4_7
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DOI: https://doi.org/10.1007/978-94-017-2858-4_7
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