Skip to main content

The Use of in Vitro Techniques for Genetic Modification of Forest Trees

  • Chapter
Tissue Culture in Forestry

Part of the book series: Forestry Sciences ((FOSC,volume 5))

Abstract

Encouraging reports of the development of techniques for in vitro mutant selection (56) and for fusion, culture and regeneration of somatic hybrid plants from protoplasts of herbaceous species (79) suggest applications of new techniques in genetic improvement programs for forest trees. In addition, through a more thorough understanding of gene expression in higher organisms (9), genetic engineering of plants, including forest species, is likely to become a reality in the near future (47). Previous reviews have addressed the topic of somatic cell genetic manipulation of plants (26, 28, 56, 72, 78, 79, 83). The present paper will discuss the use of in vitro systems for genetic modification of forest trees. It must be noted at the outset that recent contributions are few in number. Considerable additional effort must be expended before use of such techniques becomes an integral part of tree breeding programs.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. ABO El-NIL M (personal communication)

    Google Scholar 

  2. AVERY OT, CM MACLEOD, M MC CARTY 1944 Studies on the chemical nature of the substance inducing transformation of pneumonococcal type I. Induction of transformation by deoxyribonucleic acid fraction isolated from pneumonococcus type III. J Exp Med 79: 137–158

    Google Scholar 

  3. AVIV D, E GALUN 1977 An attempt at isolation of nutritional mutants from cultured tobacco protoplasts. Plant Sci Lett 8: 299–304

    Article  Google Scholar 

  4. BERG RH, M TYLER, N NOVICK, V VASIL, IK VASIL 1980 Biology of Azospirillum sugar cane association: Enhancement of nitrogenenase activity. Appl Environ Microbiol 39: 642–649

    Google Scholar 

  5. BERG RH, V VASIL, IK VASIL 1979 Biology of Azospirillum sugar cane association. II Ultrastructure. Protoplasma 101: 143–163

    Google Scholar 

  6. BERGMANN L 1960 Growth and division of single cells of higher plants in vitro. J Gen Physiol 43: 841–851

    Article  PubMed  CAS  Google Scholar 

  7. BINNS AN, F MEINS 1980 Chromosome number and the degree of cytokinin habituation of cultured tobacco pith cells. Protoplasma 103: 179–187

    Article  Google Scholar 

  8. BRIGHT SWJ, PB NORBURY, BJ MIFLIN 1979 Isolation of a recessive barley mutant resistant to S-(2 aminoethyl) L-cysteine. Theor Appl Genet 55: 1–4

    Article  CAS  Google Scholar 

  9. BROWN DD 1981 Gene expression in eukaryotes. Science 211: 667–674

    Article  PubMed  CAS  Google Scholar 

  10. BRUGOON AC, PJ BOTTINO 1976 Uptake of the nitrogen-fixing blue-green algae GZeocapsa into protoplasts of tobacco and maize. J Hered 67: 233–236

    Google Scholar 

  11. BUTENKO RG 1979 Cultivation of isolated protoplasts and hybridization of somatic plant cells. Intl Rev Cytol 59: 323–373

    Article  Google Scholar 

  12. CARLSON PS 1970 Induction and isolation of auxotrophic mutants in somatic cell cultures of Nicotiana tabaewn. Science 168: 487–489

    Article  PubMed  CAS  Google Scholar 

  13. CARLSON PS 1973 The use of protoplasts for genetic research. Proc Natl Acad Sci USA 70: 598–602

    Article  PubMed  CAS  Google Scholar 

  14. CHALEFF RS, PS CARLSON 1974 In vitro selection for mutants of higher plants. In L Ledoux, ed, Genetic Manipulations with Plant Material, Plenum Press, New York, pp 351–363

    Google Scholar 

  15. CHALEFF RS, PS CARLSON 1974 Somatic cell genetics of higher plants. Ann Rev Genet 8: 267–278

    Article  PubMed  CAS  Google Scholar 

  16. CHALEFF RS, J POLACCO 1977 In H Smith, ed, Molecular Biology of Plant Cells, University of California Press, Berkeley, pp 429–441

    Google Scholar 

  17. CHILTON MD, MN DRUMMOND, DJ MERLO, D SCIAKY, AL MONTOYA, MP GORDON, EW NESTER 1977 Stable incorporation of plasmid DNA into higher plant cells: The molecular basis of crown gall tumorigenesis. Cell 11: 263271

    Google Scholar 

  18. COCKING EC 1960 A method for the isolation of plant protoplasts and vacuoles. Nature 187: 927–929

    Article  Google Scholar 

  19. DAVEY MR 1977 Bacterial uptake and nitrogen fixation. In J Reinert, YPS Bajaj, eds, Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture, Springer, Berlin, pp 551–561

    Google Scholar 

  20. DAVEY MR, EC COCKING, J FREEMAN, N PEARCE, I TUDOR 1980 Transformation of Petunia protoplasts by isolation Agrobacterium plasmids. Plant Sci Lett 18: 307–313

    Article  CAS  Google Scholar 

  21. DAVID A, H DAVID 1979 Isolation and callus formation from cotyledon protoplasts of pine (Pinus pinaster). Z Pflanzenphysiol 94: 173–177

    CAS  Google Scholar 

  22. DIX PJ, F JOO, P MALIGA 1977 A cell line of Nicotiana syZvestris with resistance to kanamycin and streptomycin. Mol Gen Genet 157: 285–290

    Article  CAS  Google Scholar 

  23. DIX PJ, HE STREET 1976 Selection of plant cell lines with enhanced chilling resistance. Ann Bot 40: 903–910

    Google Scholar 

  24. DRUMMOND MH, MP GORDON, EW NESTER, MD CHILTON 1977 Foreign DNA of bacterial plasmid origin is transcribed in crown gall tumors. Nature 269: 535–536

    Article  CAS  Google Scholar 

  25. DUHOUX E 1980 Protoplast isolation of gymnosperm pollen. Z Pflanzenphysiol 99: 207–214

    Google Scholar 

  26. GAMBORG OL, K OHYAMA, LE PELCHER, LC FOWKE, K KARTHA, F CONSTABEL, K KAO 1979 Genetic modification of plants. In WR Sharp, PO Larsen, EF Paddock V Raghavan, eds, Plant Cell and Tissue Culture: Principles and Applications, Ohio State University Press, Columbus, pp 371–400

    Google Scholar 

  27. GILES KL 1977 Chloroplast uptake and genetic complementation. In J Reinert, YPS Bajaj, eds, Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture, Springer, Berlin, pp 536–550

    Google Scholar 

  28. GILES KL 1978 The uptake of organelles and microorganisms by plant protoplasts: Old ideas, but new horizons. In TA Thorpe, ed, Frontiers of Plant Tissue Culture, International Association for Plant Tissue Culture, Calgary, pp 67–74

    Google Scholar 

  29. GILES KL, IK VASIL 1980 Nitrogen fixation and plant tissue culture. Intl Rev Cytol Supple 11B: 81–89

    Article  Google Scholar 

  30. GILES KL, H WHITEHEAD 1976 Uptake and continued metabolic activity of Azotobacter within fungal protoplasts. Science 193: 1125–1126

    Article  PubMed  CAS  Google Scholar 

  31. GILES KL, HCM WHITEHEAD 1977 The localization of introduced Azotobacter cells within the mycelium of a modified mycorrhiza (Rhizopogon) capable of nitrogen fixation. Plant Sci Lett 10: 367–372

    Article  CAS  Google Scholar 

  32. GLEBA YY 1979 Non-chromosomal inheritance in higher plants as studied by somatic cell hybridization. In WR Sharpe, PO Larsen, EF Paddock, Raghavan, eds, Plant Cell and Tissue Culture: Principles and Applications, Ohio State University Press, Columbus, pp 775–788

    Google Scholar 

  33. GLEBA YY, RG BUTENKO, KN SYTNIK 1975 Protoplast fusion and parasexual hybridization in Nicotiana tabacum. Dokl Akad Nauk SSSR 221: 1196–1199

    Google Scholar 

  34. GORDON MP, SK FARRAND, D SCIAKY, A MONTOYA, MD CHILTON, D MERLO, EW NESTER 1977 In I Rubenstein, ed, Molecular Biology of Plants, Academic Press, New York, pp

    Google Scholar 

  35. GRESSHOFF PM 1979 Cyclohexamide resistance in Daucus carota cell cultures. Theor Appl Genet 54: 141–143

    Article  CAS  Google Scholar 

  36. HESS D 1978 Genetic effects in Petunia hybrida induced by pollination with pollen treated with lao-transducing phages. Z Pflanzenphysiol 90: 119–132

    CAS  Google Scholar 

  37. HESS D 1979 Genetic effects in Petunia hybrida induced by pollination with pollen treated with gal-transducing phages. Z Pflanzenphysiol 93: 429–436

    Google Scholar 

  38. HESS D, PM GRESSHOFF, D FIELITZ, D GLEISS 1975 Uptake of protein and bacteriophage into swelling and germinating pollen of Petunia hybrida. Z Pflanzenphysiol 74: 371–376

    Google Scholar 

  39. HESS D, G LEIPOLDT, RD ILLG 1979 Investigations on the lactose induction of beta-galactosidase activity in callus tissue cultures of Nemesia strumosa and Petunia hybrida. Z Pflanzenphysiol 94: 45–53

    CAS  Google Scholar 

  40. HESS D, H LORZ, EM WISSERT 1974 Uptake of bacterial DNA into swelling and germination pollen grains of Petunia hybrida and Nicotiana glauca. Z Pflanzenphysiol 166: 45–55

    Google Scholar 

  41. HESS D, G SCHNEIDER, H LORZ, G BLAICH 1976 Investigations on the tumor induction in Nicotiana glauca by pollen transfer of DNA isolated from Nicotiana Zangsdorfii. Z Pflanzenphysiol 77: 247–254

    CAS  Google Scholar 

  42. HUGHES BG, FG WHITE, MA SMITH 1978 Fate of bacterial DNA during uptake by barley and tobacco protoplasts. Z Pflanzenphysiol 87: 1–23

    CAS  Google Scholar 

  43. Huhtinen 0 1976 Early flowering of birch and its maintenance in plants regenerated through tissue cultures. Acta Hort 56: 243–249

    Google Scholar 

  44. HUHTINEN 0, Z YAHYAOGLU 1974 Das frühe Blühen von aus Kalluskulturen herangezorgenen Pflanzchen bei der Birke (Betula pendula Roth). Silvae Genetica 23: 32–34

    Google Scholar 

  45. HULL R, SH HOWELL 1978 Structure of the cauliflower mosaic virus genome. II. Variation in DNA structure and sequence between isolates. Virology 86: 482–493

    Google Scholar 

  46. IZHAR S, JB POWER 1979 Somatic hybridization in Petunia a male sterile cytoplasmic hybrid. Plant Sci Lett 14: 49–55

    Article  Google Scholar 

  47. KADO, CI 1980 Host-vector systems for genetic engineering of higher plant cells. In JK Setlow, A Holleander, eds, Genetic Engineering: Principles and Methods, Plenum Press, New York, pp 49–55

    Google Scholar 

  48. KAO KN, MR MICHAYLUK 1974 Method for high frequency intergeneric fusion of plant protoplasts. Planta 115: 355–367

    Article  CAS  Google Scholar 

  49. KAO KN, MR MICHAYLUK 1975 Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126: 105–110

    Article  CAS  Google Scholar 

  50. KIRBY, EG 1980 Factors affecting proliferation of protoplasts and cell cultures of Douglas-fir. In F Sala, B Parisi, R Cella, 0 Ciferri, Plant Cell Cultures: Results and Perspectives, Elsevier/North Holland, Amsterdam, pp 289–293

    Google Scholar 

  51. KIRBY EG, T-Y Cheng 1979 Colony formation from protoplasts derived from Douglas-fir cotyledons. Plant Sci Lett 14: 145–154

    Article  CAS  Google Scholar 

  52. KLEINHOFS A, R BEHKI 1977 Prospects for plant genome modification by nonconventional methods. Ann Rev Genet 11: 79–101

    Article  PubMed  CAS  Google Scholar 

  53. KLEINHOFS A, FC EDEN, MD CHILTON, AD BENDICH 1975 On the question of the integration of exogenous bacterial DNA. Proc Natl Acad Sci USA 72: 2748–2752

    Article  PubMed  CAS  Google Scholar 

  54. LEDOUX L, R HUART, M JACOBS 1971 Fate of exogenous DNA in Arabidopsis thaliana. II. Evidence for replication and preliminary results at the biological level. In L Ledoux, ed, Informative Macromolecules in Biological Systems, Elsevier/North Holland, Amsterdam, pp 159–174

    Google Scholar 

  55. LEDOUX L, R HUART, M JACOBS 1974 DNA-mediated genetic correction of thiamine-less Arabidopsis thaZiana. Nature 249: 17–19

    Article  PubMed  CAS  Google Scholar 

  56. MALIGA P 1980 Isolation, characterization and utilization of mutant cell lines in higher plants. Intl Rev Cytol Suppl 11A: 225–248

    Google Scholar 

  57. MALIGA P, G LAZAR, Z SVAB, F NAGY 1976 Transient cycloheximide resistance in a tobacco cell line. Mol Gen Genet 149: 267–271

    Article  PubMed  CAS  Google Scholar 

  58. MASTRANGELO IA, HH SMITH 1977 Selection and differentiation of aminopterin resistant cells of Datura innoxia. Plant Sci Lett 10: 171–179

    Article  CAS  Google Scholar 

  59. MATHES M, D EINSPAHR 1965 Comparison of tree growth and callus production in aspen. For Sci 11: 360–363

    Google Scholar 

  60. MATZKE AJM, M-D Chilton 1981 Site-specific insertion of genes into T-DNA of the Agrobacterium tumor-inducing plasmid: An approach to genetic engineering of higher plant cells. J Mol Appl Genet 1: 30–49

    Google Scholar 

  61. MEINS F, A BINNS 1977 Epigenetic variation of cultured somatic cells: Evidence for gradual changes in the requirement for factors promoting cell division. Proc Natl Acad Sci 74: 2928–2932

    Google Scholar 

  62. MEINS F, J LUTZ, R FOSTER 1981 Factors influencing the incidence of habituation for cytokinin on tobacco pith tissue in culture. Planta 150: 264–268

    Article  Google Scholar 

  63. MELCHERS G, G LABIB 1974 Somatic hybridization of plants by fusion of protoplasts. I. Selection of light resistant of hybrids of “Haploids,” light sensitive varieties of tobacco. Mol Gen Genet 135: 277–294

    Google Scholar 

  64. MOTT RL, RH SMELTZER, A MEHRA-PALTA, BJ ZOBEL 1977 Production of forest trees by tissue culture. Tappi 60: 62–64

    Google Scholar 

  65. MULLER AJ, R GRAFE 1978 Isolation and characterization of cell lines of Nicotiana tabaccum lacking nitrate reductase. Mol Gen Genet 161: 67–76

    Article  Google Scholar 

  66. OHIYAMA K 1978 DNA binding and uptake by nuclei isolated from plant protoplasts. Rate of single-stranded bacteriophage fd DNA. Plant Physiol 71: 515–520

    Article  Google Scholar 

  67. OHIYAMA K, OL GAMBORK, RA MILLER 1972 Uptake of exogenous DNA by plant protoplasts. Can J Bot 50: 2077–2080

    Article  Google Scholar 

  68. Ohyama K, LE Pelcher, A Schaefer, LC Fowke 1979 In vitro binding of Agrobacterium tumefaciens to plant cells from suspension culture. Plant Physiol 63:382–387

    Google Scholar 

  69. OHTA Y, PV CHUONG 1976 Heredity changes in Capsicum annuum L I Induced by ordinary grafting. Euphytica 24: 355–368

    Article  Google Scholar 

  70. PANDEY KK 1976 Genetic transformation and “graft hybridization” in flowering plants. Theor Appl Genet 47: 299–302

    Article  Google Scholar 

  71. PANDY KK 1978 Novel techniques of gene transfer and plant improvement: An appraisal of transformation in eukaryotes. New Phytol 81: 685–704

    Article  Google Scholar 

  72. PARKE D, PS CARLSON 1979 Somatic cell genetics of higher plants: appraising the application of bacterial systems to higher plant cells cultured in vitro. In J Scandalios, ed, Physiological Genetics, Academic Press, New York, pp 196–238

    Google Scholar 

  73. POWER JB, EC COCKING 1977 Selection systems for somatic hybrids. In J Reinert, YPS Bajaj, eds, Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture, Springer, Berlin, pp 497–505

    Google Scholar 

  74. REDEI GP 1974 Induction of auxotrophic mutations in plants. In L Ledoux, ed, Genetic Manipulations with Plant Material, Plenum Press, New York, pp 329–350

    Google Scholar 

  75. REDENBAUGH MK, RD WESTFALL, DF KARNOSKY 1980 Protoplast isolation from UZmus americana L. pollen mother cells, tetrads and microspores. Can J For 10: 284–289

    Google Scholar 

  76. SCHELL J, M VAN MONTAGU 1978 Transfer, maintenance and expression of bacterial Ti plasmid DNA in plant cells transformed with A. tumefaciens. Brookhaven Symp Biol 27: 36–49

    Google Scholar 

  77. SCHELL J, M Van MONTAGU, M DE BEUCKELEER, M DE BLOCK, A DEPICKER, M DE WILDE, G ENGLER, C GENETELLO, JP HARNALSTEENS, M HOLSTERS, J SEURINCK, B SILVA, F Van VLIET, R VILLARROEL 1979 Interactions and DNA transfer between Agrobacterium tumafaciens, the Ti plasmid and the plant host. Proc Royal Soc Lond (Biol) 204: 251–266

    Article  CAS  Google Scholar 

  78. SCHEIDER, 0 1978 Production and uses of metabolic and chlorophyll deficient mutants. In TA Thorpe, ed, Frontiers of Plant Tissue Culture, International Association for Plant Tissue Culture, Calgary, pp 393–401

    Google Scholar 

  79. SCHIEDER 0, IK VASIL 1980 Protoplast fusion and somatic hybridization. Intl Rev Cytol Suppl 11B: 21–46

    Article  Google Scholar 

  80. SHEPHERD RJ 1979 DNA plant viruses. Ann Rev Plant Physiol 30: 405–423

    Article  CAS  Google Scholar 

  81. SHEPARD JF, D BIONEY, E SHAHIN 1980 Potato protoplasts in crop improvement. Science 208: 17–24

    Article  PubMed  CAS  Google Scholar 

  82. SHVIDKAYA S, Y GLEBA 1974 Plating of mesophyll protoplasts isolated from different photosynthetic mutants of Nicotiana tabacum. Abstr #195, 3rd Internatl Congress Plant Tissue and Cell Culture, Leicester, UK

    Google Scholar 

  83. SMITH HH, IA MASTRANGELO-HOUGH 1979 Genetic variability available through cell fusion. In WR Sharpe, PO Larsen, EF Paddock, V Raghavan, eds, Plant Cell and Tissue Culture: Principles and Applications, Ohio State University Press, Columbus, pp 265–286

    Google Scholar 

  84. STENT GS, R CALENDAR 1978 Molecular Genetics, Ed 2, WH Freeman and Co, San Francisco

    Google Scholar 

  85. SZETO WW, DH HAMMER, PS CARLSON, CA THOMAS 1977 Cloning of cauliflower mosaic virus (CLMV) in Escherichia coli. Science 196: 210–212

    Article  PubMed  CAS  Google Scholar 

  86. TAKAI S 1974 Pathogenecity and cerato-ulmin production in Ceratocystis ulmi. Nature 252: 124–126

    Article  PubMed  CAS  Google Scholar 

  87. TUMANOV II, RG BUTENKO, IV OGOLEVETS, VV SMETZUK 1977 Frost resistance in the culture of spruce callus tissue increased by freezing less resistant cells. Fiziol Rast (Moscow) 24: 895–900

    Google Scholar 

  88. UCHIMIYA H, T MURASHIGE 1977 Quantitative analysis of the fate of exogenous DNA in Nicotiana protoplasts. Plant Physiol 59: 301–308

    Article  PubMed  CAS  Google Scholar 

  89. VASIL IK, V VASIL 1980 Isolation and culture of protoplasts. Intl Rev Cytol Suppl 11B: 1–20

    Article  Google Scholar 

  90. WEBER G, KG LARK 1979 An efficient plating system for rapid isolation of mutants from plant cell suspensions. Theor Appl Genet 55: 81–86

    Article  Google Scholar 

  91. WIDHOLM, JM 1974 Selection and characteristics of biochemical mutants and cultured plant cells. In HE Street, ed, Tissue Culture and Plant Science, Academic Press, New York, pp 287–299

    Google Scholar 

  92. WIDHOLM JM 1978 Selection and characterization of a Daucus carota L. cell line resistant to four amino acid analogs. J Exp Bot 29: 1111–1116

    Article  CAS  Google Scholar 

  93. WINTON LL, RA PARHAM, MA JOHNSON, DW EINSPAHR 1974 Tree improvement by callus, cell and protoplast culture. Tappi 57: 151–152

    Google Scholar 

  94. WRIGHT, JW 1976 Introduction to Forest Genetics, Academic Press, New York

    Google Scholar 

  95. ZAMBRYSKI P, M HOLSTER, K KRUGER, A DEPICKER, J SCHELL, M Van MONTAGU, HM GOODMAN 1980 Tumor DNA structure in plant cells transformed by A. tumefaciens. Science 209: 1385–1391

    Article  PubMed  CAS  Google Scholar 

  96. ZELCHER A, D AVIV, E GALUN 1978 Interspecific transfer of cytoplasmic malc sterility by fusion between protoplasts of normal Nicotiana sylvestres and x-ray irradiated protoplasts of male sterile N. tabacum. Z Pflanzenphysiol 90: 397–407

    Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1982 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Kirby, E.G. (1982). The Use of in Vitro Techniques for Genetic Modification of Forest Trees. In: Bonga, J.M., Durzan, D.J. (eds) Tissue Culture in Forestry. Forestry Sciences, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3538-4_12

Download citation

  • DOI: https://doi.org/10.1007/978-94-017-3538-4_12

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-8272-5

  • Online ISBN: 978-94-017-3538-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics