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High Resolution AFM and Its Applications

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Atomic Force Microscopy in Molecular and Cell Biology

Abstract

Imaging fine structures of molecules and cells under controlled conditions, and early detection of subtle changes in material properties are very important for understanding the mechanism of many biological processes. In the past decades, scientists made extensive efforts in exploring and developing new imaging technologies to improve the resolution and sensitivity of detection. AFM is not an exception, improving the resolution of measurements is a long-lasting effort for both users and instrument makers. It is even more challenging for soft materials, such as biological molecules, cells and biomaterials, as the force control in soft material measurement is more critical and difficult. In this chapter, we will first review the applications of high resolution AFM, discuss the challenges for high resolution in different aspects and followed by solutions to achieve high resolution for different applications, including AFM system optimization and sample-probe interaction optimization by probe design and control algorithm. A series of examples will be used as case studies to illustrate how to achieve high resolution in AFM experiments. At the end of this chapter, we will provide our prospective view on future developments in AFM technology.

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  1. Bruker Nano Surface, Singapore, Singapore

    Hao Sun, Ming Ye & Wanxin Sun

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Correspondence to Wanxin Sun .

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  1. State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China

    Jiye Cai

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Sun, H., Ye, M., Sun, W. (2018). High Resolution AFM and Its Applications. In: Cai, J. (eds) Atomic Force Microscopy in Molecular and Cell Biology. Springer, Singapore. https://doi.org/10.1007/978-981-13-1510-7_10

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