Skip to main content
SpringerLink
Log in

Aerobic Denitrification Characteristics and Mechanism of Pseudomonas stutzeri PCN-1

  • Chapter
  • First Online:
Nitrogen Removal Characteristics of Aerobic Denitrifying Bacteria and Their Applications in Nitrogen Oxides Emission Mitigation

Part of the book series: Springer Theses ((Springer Theses))

  • 388 Accesses

Abstract

More attention has been paid on the phenomenon of NO and N2O accumulation in the process of denitrification by the researchers. For example, the typical aerobic denitrifying bacterium P. denitrificans LMD 92.63 produced only N2O under the condition of a saturated dissolved oxygen concentration of 95% (Arts et al. 1995); strain P. denitrificans JCM 20620 released N2O as the final denitrification product as long as O2 is present (Miyahara et al. 2010);N2O had been produced by the strain Alcaligenes faecalis TUD, which accounted for 5% of the total nitrogen removal under the condition of 5% saturation dissolved oxygen concentration (Otte et al. 1996). In recent years, a number of aerobic denitrifying bacteria have been successfully separated, but the quantitative analysis of accumulation of NO and N2O has rarely been reported (Wan et al. 2011; Yang et al. 2011; Yao et al. 2013).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Alinsafi, A., Adouani, N., Beline, F., Lendormi, T., Limousy, L., & Sire, O. (2008). Nitrite effect on nitrous oxide emission from denitrifying activated sludge. Process Biochemistry, 43, 683–689.

    Article  CAS  Google Scholar 

  • Arts, P. A. M., Robertson, L. A., & Kuenen, J. G. (1995). Nitrification and denitrification by Thisphaera pantotropha in aerobic chemostat cultures. FEMS Microbiology Ecology, 18, 305–316.

    Article  CAS  Google Scholar 

  • Bergaust, L., Shapleigh, J., Frostegard, A., & Bakken, L. (2008). Transcription and activities of NOx reductases in Agrobacterium tumefaciens: The influence of nitrate, nitrite and oxygen availability. Environmental Microbiology, 10, 3070–3081.

    Article  CAS  Google Scholar 

  • Cabiscol, E., Tamarit, J., & Ros, J. (2000). Oxidative stress in bacteria and protein damage by reactive oxygen species. International Microbiology, 3, 3–8.

    CAS  Google Scholar 

  • Chen, J. W., & Strous, M. (2013). Denitrification and aerobic respiration, hybrid electron transport chains and co-evolution. Biochimica et Biophysica Acta, 1827, 136–144.

    Article  CAS  Google Scholar 

  • Chen, P. Z., Li, J., Li, Q. X., Wang, Y. C., Li, S. P., Ren, T. Z., & Wang, L. G. (2012). Simultaneous heterotrophic nitrification and aerobic denitrification by bacterium Rhodococcus sp. CPZ24. Bioresource Technology, 116, 266–270.

    Article  CAS  Google Scholar 

  • Guo, L. Y., Chen, Q. K., Fang, F., Hu, Z. X., Wu, J., Miao, A. J., Xiao, L., Chen, X. F., & Yang, L. Y. (2013). Application potential of a newly isolated indigenous aerobic denitrifier for nitrate and ammonium removal of eutrophic lake water. Bioresource Technology, 142, 45–51.

    Article  CAS  Google Scholar 

  • Hartig, E., & Zumft, W. G. (1999). Kinetics of nirS expression (cytochrome cd1 nitrite reductase) in Pseudomonas stutzeri during the transition from aerobic respiration to denitrification: Evidence for a denitrification-specific nitrate- and nitrite-responsive regulatory system. Journal of Bacteriology, 181, 161–166.

    CAS  Google Scholar 

  • Kester, R. A., De Boer, W., & Laanbroek, H. J. (1997). Production of NO and N2O by pure cultures of nitrifying and denitrifying bacteria during changes in aeration. Applied and Environmental Microbiology, 63, 3872–3877.

    CAS  Google Scholar 

  • Miyahara, M., Kim, S. W., Fushinobu, S., Takaki, K., Yamada, T., Watanabe, A., Miyauchi, K., & Endo, G. (2010). Potential of aerobic denitrification by Pseudomonas stutzeri TR2 to reduce nitrous oxide emissions from wastewater treatment plants. Applied and Environmental Microbiology, 76, 4619–4625.

    Article  CAS  Google Scholar 

  • Muyzer, G., De Waal, E. C., & Uitterlinden, A. G. (1993). Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Applied and Environmental Microbiology, 59, 695–700.

    CAS  Google Scholar 

  • Otte, S., Grobben, N. G., Robertson, L. A., Jetten, M. S., & Kuenen, J. G. (1996). Nitrous oxide production by Alcaligenes faecalis under transient and dynamic aerobic and anaerobic conditions. Applied and Environmental Microbiology, 62, 2421–2426.

    CAS  Google Scholar 

  • Philippot, L. (2002). Denitrifying genes in bacterial and archaeal genomes. Biochimica et Biophysica Acta, 1577, 355–376.

    Article  CAS  Google Scholar 

  • Pomowski, A., Zumft, W. G., Kroneck, P. M. H., & Einsle, O. (2011). N2O binding at a [4Cu:2S] copper-sulphur cluster in nitrous oxide reductase. Nature, 477, 234–237.

    Article  CAS  Google Scholar 

  • Poole, R. K., & Hughes, M. N. (2000). New functions for the ancient globin family: Bacterial responses to nitric oxide and nitrosative stress. Molecular Microbiology, 36, 775–783.

    Article  CAS  Google Scholar 

  • Throback, I. N., Enwall, K., Jarvis, A., & Hallin, S. (2004). Reassessing PCR primers targeting nirS, nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE. FEMS Microbiology Ecology, 49, 401–417.

    Article  CAS  Google Scholar 

  • Saleh-Lakha, S., Shannon, K. E., Goyer, C., Trevors, J. T., Zebarth, B. J., & Burton, D. L. (2008). Nitric oxide reductase gene expression and nitrous oxide production in nitrate-grown Pseudomonas mandelii. Applied and Environmental Microbiology, 74, 6876–6879.

    Article  CAS  Google Scholar 

  • Spiro, S. (2012). Nitrous oxide production and consumption: Regulation of gene expression by gas-sensitive transcription factors. Philosophical Transactions of the Royal Society B, 367, 1213–1225.

    Article  CAS  Google Scholar 

  • Wan, C. L., Yang, X., Lee, D. J., Du, M. A., Wan, F., & Chen, C. (2011). Aerobic denitrification by novel isolated strain using NO2−-N as nitrogen source. Bioresource Technology, 102, 7244–7248.

    Article  CAS  Google Scholar 

  • Wunderlin, P., Mohn, J., Joss, A., Emmenegger, L., & Siegrist, H. (2012). Mechanisms of N2O production in biological wastewater treatment under nitrifying and denitrifying conditions. Water Research, 46, 1027–1037.

    Article  CAS  Google Scholar 

  • Yang, X. P., Wang, S. M., Zhang, D. W., & Zhou, L. X. (2011). Isolation and nitrogen removal characteristics of an aerobic heterotrophic nitrifying-denitrifying bacterium, Bacillus subtilis A1. Bioresource Technology, 102, 854–862.

    Article  CAS  Google Scholar 

  • Yao, S., Ni, J. R., Ma, T., & Li, C. (2013). Heterotrophic nitrification and aerobic denitrification at low temperature by a newly isolated bacterium, Acinetobacter sp. HA2. Bioresource Technology, 139, 80–86.

    Article  CAS  Google Scholar 

  • Zhang, Q. L., Liu, Y., Ai, G. M., Miao, L. L., Zheng, H. Y., & Liu, Z. P. (2012a). The characteristics of a novel heterotrophic nitrification-aerobic denitrification bacterium, Bacillus methylotrophicus strain L7. Bioresource Technology, 108, 35–44.

    Article  CAS  Google Scholar 

  • Zhang, T., Shao, M. F., & Ye, L. (2012b). 454 pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants. ISME Journal, 6, 1137–1147.

    Article  CAS  Google Scholar 

  • Zheng, M. S., He, D., Ma, T., Chen, Q., Liu, S. T., Ahmad, M., Gui, M. Y., & Ni, J. R. (2014). Reducing NO and N2O emission during aerobic denitrification by newly isolated Pseudomonas stutzeri PCN-1. Bioresource Technology, 162, 80–88.

    Article  CAS  Google Scholar 

  • Zumft, W. G. (1997). Cell biology and molecular basis of denitrification. Microbiology and Molecular Biology Reviews, 61, 533–616.

    CAS  Google Scholar 

  • Zweier, J. L., Wang, P., Samouilov, A., & Kuppusamy, P. (1995). Enzyme-independent formation of nitric oxide in biological tissues. Nature Medicine, 1, 804–809.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Environmental Science and Engineering, MOE Key Laboratory of Resources and Environmental Systems Optimization, North China Electric Power University, Beijing, China

    Dr. Maosheng Zheng

Authors
  1. Dr. Maosheng Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Zheng, M. (2019). Aerobic Denitrification Characteristics and Mechanism of Pseudomonas stutzeri PCN-1. In: Nitrogen Removal Characteristics of Aerobic Denitrifying Bacteria and Their Applications in Nitrogen Oxides Emission Mitigation. Springer Theses. Springer, Singapore. https://doi.org/10.1007/978-981-13-2432-1_3

Download citation

Publish with us

Policies and ethics

Search

Navigation