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Effects of Space Microgravity on the Trans-differentiation Between Osteogenesis and Adipogenesis of Human Marrow-Derived Mesenchymal Stem Cells

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Life Science in Space: Experiments on Board the SJ-10 Recoverable Satellite

Part of the book series: Research for Development ((REDE))

Abstract

With the development of scientific exploration in deep space, human activities will become more frequent, and activity time will be longer in the deep space. The environment alteration may cause severe bone changes of human in deep space. The changes of bone mass caused by spatial microgravity are related to the decrease of osteoblast formation and development in bone tissue, and the decrease of osteoblast formation is related to the down-regulation of differentiation of human bone marrow mesenchymal stem cells (hMSCs). Therefore, the study for the biological effects of microgravity on bone cell formation and the relative molecular mechanisms at stem cell level is one of the important subjects to explore the effects of spatial microgravity on bone changes. These studies may provide a scientific basis for the development and the related technologies of target drugs research. Based on exploring the flight conditions on the ground and simulating flight experiments with the automated space experimental device, we utilized a real microgravity environment in the SJ-10 recoverable microgravity experimental satellite (SJ-10 satellite) to examine the effects of space microgravity on transcriptome expression and differentiation potentials of hMSCs.

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Abbreviations

ALP:

Alkaline phosphatase

BMP2:

Bone morphogenetic protein-2

ECM:

Extracellular matrix

FBS:

Heat-inactivated fetal bovine serum

GADD45:

Growth arrest and DNA damage inducible alpha

GO:

Gene ontology

hBMSCs:

Human bone marrow mesenchymal stem cells

LSM:

Lymphocyte separation medium

MMP1:

Matrix metallopeptidase 1

MSCs:

Mesenchymal stem cells

NG:

Normal ground gravity

PBS:

Phosphate buffer saline

PLGA:

Synthetic poly (D, L-lactide-co-glycolide)

qRT-PCR:

Quantitative real-time PCR

RNA-SEQ:

RNA sequence

RPM:

Random positioning machine

SEM:

Scanning electronic microscope

SMG:

Simulated microgravity

α-MEM:

Α-minimum essential medium

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Acknowledgements

We much thank the opportunity of space experiment supplied by SJ-10 Recoverable Scientific Satellite. This study was supported by the grants from Strategically Guiding Scientific Special Project from Chinese Academy of Sciences (XDA04020202-23), Chinese National Nature Science Foundation (U1738102, 81570932).

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Authors and Affiliations

  1. Institute of Cell and Development Biology, College of Life Sciences, Zhejiang University, Zijingang Campus, Hangzhou, 310058, Zhejiang, People’s Republic of China

    Cui Zhang & Liang Li

  2. College of Life Sciences, Zhejiang University, Zijingang Campus, Number 866 of Yuhangtang Road, West Lake Dist, Hangzhou, Zhejiang, People’s Republic of China

    Jinfu Wang

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  2. Liang Li
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  3. Jinfu Wang
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Correspondence to Jinfu Wang .

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Editors and Affiliations

  1. Institute of Zoology, Chinese Academy of Sciences, Beijing, China

    Enkui Duan

  2. Institute of Mechanics, Chinese Academy of Sciences, Beijing, China

    Mian Long

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Zhang, C., Li, L., Wang, J. (2019). Effects of Space Microgravity on the Trans-differentiation Between Osteogenesis and Adipogenesis of Human Marrow-Derived Mesenchymal Stem Cells. In: Duan, E., Long, M. (eds) Life Science in Space: Experiments on Board the SJ-10 Recoverable Satellite. Research for Development. Springer, Singapore. https://doi.org/10.1007/978-981-13-6325-2_12

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