Abstract
Urine is directly connected to the urinary system; as a result, urine proteome was usually used for renal diseases. As urine accumulates all types of changes, identifying the precise cause of changes in the urine proteome is challenging and crucial in biomarker discovery. To reduce the confounding factors to minimal, some studies used animal model resembling human diseases. This chapter highlights the importance of animal models and introduces two strategic researches which focused on serial changes of urine proteome in animal model of focal segmental glomerulosclerosis and tubular injury. In these studies, urine samples were collected at different stages of animal models, and urinary proteins were profiled by LC-MS/MS. For the focal segmental glomerulosclerosis model, 25 urinary proteins changed in the whole process. For the unilateral ureteral obstruction model, 7 and 19 significantly changed in the 1- and 3-week groups, respectively. We think these stage-dependent dynamic changes of urine proteome in animal models will help to support the role of urine as key source in biomarker discovery especially in kidney diseases and help to identify corresponding biomarkers for clinical validation.
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References
Adachi J, Kumar C, Zhang Y, Olsen JV, Mann M. The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins. Genome Biol. 2006;7:R80.
Chevalier RL, Forbes MS, Thornhill BA. Ureteral obstruction as a model of renal interstitial fibrosis and obstructive nephropathy. Kidney Int. 2009;75:1145–52.
Decramer S, Gonzalez de Peredo A, Breuil B, Mischak H, et al. Urine in clinical proteomics. Mol Cell proteomics. 2008;7:1850–62.
Grande MT, Lopez-Novoa JM. Fibroblast activation and myofibroblast generation in obstructive nephropathy. Nat Rev Nephrol. 2009;5:319–28.
Haynes PA, Yates JR 3rd. Proteome profiling-pitfalls and progress. Yeast. 2000;17:81–7.
Jia L, Zhang L, Shao C, Song E, et al. An attempt to understand kidney’s protein handling function by comparing plasma and urine proteomes. PLoS One. 2009;4:e5146.
Jia L, Li X, Shao C, Wei L, et al. Using an isolated rat kidney model to identify kidney origin proteins in urine. PLoS One. 2013;8:e66911.
Kandasamy N, Ashokkumar N. Protective effect of bioflavonoid myricetin enhances carbohydrate metabolic enzymes and insulin signaling molecules in streptozotocin-cadmium induced diabetic nephrotoxic rats. Toxicol Appl Pharmacol. 2014;279:173–85.
Kitiyakara C, Kopp JB, Eggers P. Trends in the epidemiology of focal segmental glomerulosclerosis. Semin Nephrol. 2003;23:172–82.
Klein J, Schanstra JP. Implementation of proteomics biomarkers in nephrology: from animal models to human application? Proteomics Clin Appl. 2018:e1800089.
Kriz W, Gretz N, Lemley KV. Progression of glomerular diseases: is the podocyte the culprit? Kidney Int. 1998;54:687–97.
Li QR, Fan KX, Li RX, Dai J, et al. A comprehensive and non-prefractionation on the protein level approach for the human urinary proteome: touching phosphorylation in urine. Rapid Commun Mass Spectrom. 2010;24:823–32.
Marimuthu A, O’Meally RN, Chaerkady R, Subbannayya Y, et al. A comprehensive map of the human urinary proteome. J Proteome Res. 2011;10:2734–43.
Minarowska A, Minarowski L, Karwowska A, Sands D, Dabrowska E. The activity of cathepsin D in saliva of cystic fibrosis patients. Folia Histochem Cytobiol. 2007;45:165–8.
Moles A, Tarrats N, Fernandez-Checa JC, Mari M. Cathepsins B and D drive hepatic stellate cell proliferation and promote their fibrogenic potential. Hepatology. 2009;49:1297–307.
Ngai HH, Sit WH, Jiang PP, Xu RJ, et al. Serial changes in urinary proteome profile of membranous nephropathy: implications for pathophysiology and biomarker discovery. J Proteome Res. 2006;5:3038–47.
Pippin JW, Brinkkoetter PT, Cormack-Aboud FC, Durvasula RV, et al. Inducible rodent models of acquired podocyte diseases. Am J Physiol Renal Physiol. 2009;296:F213–29.
Rampanelli E, Rouschop KM, Claessen N, Teske GJ, et al. Opposite role of CD44-standard and CD44-variant-3 in tubular injury and development of renal fibrosis during chronic obstructive nephropathy. Kidney Int. 2014;86:558–69.
Saha S, Harrison SH, Shen C, Tang H, et al. HIP2: an online database of human plasma proteins from healthy individuals. BMC Med Genet. 2008;1:12.
Schaffer P, Molnar L, Lukasz P, Mattyus I, et al. Urinary enzyme excretion in childhood uropathy. Orv Hetil. 2002;143:2135–9.
Shao C, Li M, Li X, Wei L, et al. A tool for biomarker discovery in the urinary proteome: a manually curated human and animal urine protein biomarker database. Mol Cell Proteomics. 2011;10:M111.010975.
Sharma V, Tikoo K. Stage-specific quantitative changes in renal and urinary proteome during the progression and development of streptozotocin-induced diabetic nephropathy in rats. Mol Cell Biochem. 2014;388:95–111.
Silverstein DM, Travis BR, Thornhill BA, Schurr JS, et al. Altered expression of immune modulator and structural genes in neonatal unilateral ureteral obstruction. Kidney Int. 2003;64:25–35.
Tampe B, Zeisberg M. Contribution of genetics and epigenetics to progression of kidney fibrosis. Nephrol Dial Transplant. 2014;29(Suppl 4):iv72–9.
Thomas DB. Focal segmental glomerulosclerosis: a morphologic diagnosis in evolution. Arch Pathol Lab Med. 2009;133:217–23.
Wang Y, Chen Y, Zhang Y, Wu S, et al. Differential ConA-enriched urinary proteome in rat experimental glomerular diseases. Biochem Biophys Res Commun. 2008;371:385–90.
Yuan Y, Zhang F, Wu J, Shao C, Gao Y. Urinary candidate biomarker discovery in a rat unilateral ureteral obstruction model. Sci Rep. 2015;5:9314.
Zhao M, Li M, Li X, Shao C, et al. Dynamic changes of urinary proteins in a focal segmental glomerulosclerosis rat model. Proteome Sci. 2014;12:42.
Acknowledgment
A part of this chapter is reused with permission from our previous published book chapter, Zhao M. (2015) Human Urine Proteome: A Powerful Source for Clinical Research. In: Gao Y. (eds) Urine Proteomics in Kidney Disease Biomarker Discovery. Advances in Experimental Medicine and Biology, vol 845. Springer, Dordrecht, and our previous published paper, Yuan, Yuan Y…Gao Y. “Urinary candidate biomarker discovery in a rat unilateral ureteral obstruction model,” Scientific Reports, 2015.
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Zhao, M., Yuan, Y. (2019). Serial Changes of Urinary Proteome in Animal Models of Renal Diseases. In: Gao, Y. (eds) Urine. Springer, Singapore. https://doi.org/10.1007/978-981-13-9109-5_17
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DOI: https://doi.org/10.1007/978-981-13-9109-5_17
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