Abstract
Hairy root cultures are widely used for the production of different secondary metabolites, since their level of synthesis in transgenic roots is often higher than that of non-transgenic tissues. However, the mechanism of influence of T-DNA genes on plant metabolism is not studied well. We offer a diagnostic panel for Fluidigm BioMark for investigation of the expression of genes associated with the synthesis of alkaloids in tobacco hairy roots and related PCR protocol. The list of investigated genes includes genes of alkaloid biosynthesis, genes of transcription factors participating in the regulation of alkaloid content, rol genes, and reference genes.
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Acknowledgment
The authors are grateful to RC Development of Molecular and Cellular Technologies of Saint Petersburg State University for providing the necessary facilities to carry out the work. Grant 18-016-00118 provided by Russian Foundation for Basic research for TM is acknowledged.
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Matveeva, T., Amini, G. (2020). Fluidigm BioMark Diagnostic Panel for Analysis of the Expression of Nicotiana tabacum Genes, Associated with Alkaloid Synthesis. In: Srivastava, V., Mehrotra, S., Mishra, S. (eds) Hairy Root Cultures Based Applications. Rhizosphere Biology. Springer, Singapore. https://doi.org/10.1007/978-981-15-4055-4_15
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DOI: https://doi.org/10.1007/978-981-15-4055-4_15
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