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Structural features of aquatic fulvic acids by analytical and preparative HPLC followed by spectroscopic characterization

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Humic Substances in the Aquatic and Terrestrial Environment

Part of the book series: Lecture Notes in Earth Sciences ((LNEARTH,volume 33))

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Abstract

Analytical and preparative reversed-phase liquid chromatography were used to separate constituents of Suwannee River aquatic fulvic acid. Photodiode array UV-Vis, and fluorescence detectors were used to monitor the HPLC separation. Preparative FA fractions were subjected to 1H and 13C solid state NMR, FT-IR, and ESR. The preparative and analytical RP-HPLC separations have shown that the FA macromolecule consists of at least two polymeric fractions, each of which can further be separated into individual constituents by analytical RP-HPLC. Vanillic acid structures were identified in the analytical chromatograms of the total FA sample, but not in the fractions. The first fraction included the hydrophilic constituents of FA and represented about 40 percent of the total sample. It could be resolved into six constituents by analytical RP-HPLC. Their tR and UV-Vis scans were characteristic of carboxylic acids in rigid structures which exhibit strong hydrogen bonding. The second fraction included the hydrophobic constituents and represented about 30 percent of the total sample. It could be resolved into twelve constituents. Repeated structurally related units were separated and their UV-Vis scans were characteristic of conjugated ketone and phenolic structures. Free radical structures were present in both hydrophilic and hydrophobic fractions.

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Authors and Affiliations

  1. Institute of Applied Sciences and Department of Chemistry, University of North Texas, 76203, Denton, Texas, USA

    Farida Y. Saleh, Wenching A. Ong, Inyoung Kim & Qasem Haj-Mahmoud

Authors
  1. Farida Y. Saleh
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  2. Wenching A. Ong
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  3. Inyoung Kim
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  4. Qasem Haj-Mahmoud
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Bert Allard Hans Borén Anders Grimvall

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© 1991 Springer-Verlag

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Saleh, F.Y., Ong, W.A., Kim, I., Haj-Mahmoud, Q. (1991). Structural features of aquatic fulvic acids by analytical and preparative HPLC followed by spectroscopic characterization. In: Allard, B., Borén, H., Grimvall, A. (eds) Humic Substances in the Aquatic and Terrestrial Environment. Lecture Notes in Earth Sciences, vol 33. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0010457

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  • DOI: https://doi.org/10.1007/BFb0010457

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-53702-1

  • Online ISBN: 978-3-540-46985-8

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