Abstract
A linear DNA can be dissolved in nonaqueous low-polar organic solvents by forming complexes between DNA and cationic surfactants. The data of high-rate sedimentation, UV-spectrometric and circular dichroism measurements show that the DNA complexes with surfactant in chloroform, heptane and cyclohexane are individual components with a 1∶1 stoichiometry. The DNA molecules passing to the organic phase retain a double-stranded helix conformation. By means of scanning tunneling and atomic force microscopy it was found that DNA-surfactant complexes in water and in chloroform exist in compact toroidal conformation. It was demonstrated that such complexes can be transferred through water/organic-phase boundaries.
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© 1997 Steinkopff Verlag
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Sergeyev, V.G., Pyshkina, O.A., Gallyamov, M.O., Yaminsky, I.V., Zezin, A.B., Kabanov, V.A. (1997). DNA-surfactant complexes in organic media. In: Kawasaki, K., Lindman, B., Okabayashi, H. (eds) Formation and Dynamics of Self-Organized Structures in Surfactants and Polymer Solutions. Progress in Colloid & Polymer Science, vol 106. Steinkopff. https://doi.org/10.1007/BFb0111058
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DOI: https://doi.org/10.1007/BFb0111058
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