Disintegration of poly ({ie133-1}-L-lysinidylterephthaloyl) microcapsules by fibrinogen

Abstract

Poly({ie133-2}-L-lysinediylterephthaloyl) (abbreviation: PPL) microcapsules were prepared by an interfacial polymerization technique which makes use of the interfacial polycondensation reaction between L-lysine dissolved in water and terephthaloyl dichloride dissolved in an organic solvent. Interaction of PPL microcapsules with fibrinogen was investigated as a function of fibrinogen concentration, pH and ionic strength of the medium, and anion species of added salt.

PPL microcapsules were found to undergo disintegration by the action of fibrinogen molecules at high ionic strengths of the medium, irrespective of pH. The presence of thiocyanate ion, a breaker of water structure, enhanced disintegration of PPL microcapsules by fibrinogen while citrate ion, a maker of water structure, suppressed it. It was shown fluometrically by the use of dansylated fibrinogen that PPL microcapsules adsorb fibrinogen molecules prior to their disintegration.

Disintegration of PPL microcapsules by fibrinogen was suggested to be caused by the hydrophobic interaction of PPL molecules with fibrinogen molecules, leading to intermingling of the polymers involved, based on the experimental findings.