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DNA Adsorption on Carbonaceous Materials

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Immobilisation of DNA on Chips I

Part of the book series: Topics in Current Chemistry ((TOPCURRCHEM,volume 260))

Abstract

The immobilization of DNA on different solid supports has become an important issue in different fields ranging from medicine to analytical chemistry and, more recently, molecular electronics. Among the different immobilization procedures, adsorption is the simplest and the easiest to automate, avoiding the use of procedures based on previous activation/modification of the substrate and subsequent immobilization, which are tedious, expensive and time-consuming. Carbon-based materials are widely used for this task due to their electrochemical, physical and mechanical properties, their commercial availability, and their compatibility with modern microchip fabrication technology. Moreover, carbonaceous materials are widely used as transducers for electrochemical sensors. The knowledge of the adsorbed DNA morphology on carbon surfaces can be used to develop stable and functional DNA layers for their use in DNA analytical devices with improved properties. Presented here is a concise description of surface immobilization of DNA, oligonucleotides, and DNA derivatives by adsorption onto carbonaceous materials, and the properties of the DNA layer adsorbed on carbonaceous solid phase.

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Abbreviations

A:

Adenine

ABS:

Acetate buffer solution

AFM:

Atomic force microscopy

BDD:

Boron-doped diamond

BLM:

Bilayer lipid membrane

C:

Cytosine

CNT:

Carbon nanotube

CNTP:

Carbon nanotube paste

CP:

Carbon paste

dsDNA:

Double-stranded DNA or native DNA

G:

Guanine

GC:

Glassy carbon

GC(ox) :

Anodized glassy carbon

GEC:

Graphite epoxy composite

HOPG:

Highly ordered pyrolytic graphite

MWCNT:

Multi-wall carbon nanotube

ODN:

Oligodeoxynucleotide

PBS:

Phosphate buffer solution

PG:

Pyrolytic graphite

SCE:

Saturated calomel electrode

ssDNA:

Single-stranded DNA or denatured DNA

SWCNT:

Single-wall carbon nanotube

T:

Thymine

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Authors and Affiliations

  1. Grup de Sensors i Biosensors, Departament de Química, Universitat Autónoma de Barcelona, Barcelona, Spain

    María Isabel Pividori & Salvador Alegret

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  1. María Isabel Pividori
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  2. Salvador Alegret
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Correspondence to María Isabel Pividori .

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Christine Wittmann

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Pividori, M.I., Alegret, S. DNA Adsorption on Carbonaceous Materials. In: Wittmann, C. (eds) Immobilisation of DNA on Chips I. Topics in Current Chemistry, vol 260. Springer, Berlin, Heidelberg. https://doi.org/10.1007/b136064

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